Diverse accumulation and distribution of nutrient elements in developing wheat grain studied by laser ablation inductively coupled plasma mass spectrometry imaging.
Diverse accumulation and distribution of nutrient elements in developing wheat grain studied by laser ablation inductively coupled plasma mass spectrometry imaging.
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DOI：
10.1039/c3mt00071k
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发表时间：
2013-08
期刊：
影响因子：
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通讯作者：
B. Wu;Franka Andersch;W. Weschke;H. Weber;J. Sabine Becker
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文献类型：
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作者：
B. Wu;Franka Andersch;W. Weschke;H. Weber;J. Sabine Becker
利用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）成像技术，研究了小麦籽粒发育过程中元素的分布。结果表明，小麦胚中积累了大量的Fe、K、Cu、Zn等营养元素，而小麦麸皮中积累了大量的Ca，这可能与其结构维持功能有关。胚乳中的营养物质主要分布在糊粉层中。在籽粒中，胚可以被认为是幼苗发育所必需的宏量和微量元素的营养库。元素图像显示，胚胎的盾片中储存了相当数量的营养物质，这可能与盾片中元素转运蛋白基因的高表达有关。根原基和叶原基分别富集Mn和Zn等特殊元素。总共分析了34个横截面，并用于生成一系列元素分布图像，以展示元素沿小麦粒胚垂直轴的沿着变化。三维建模的进一步发展将与生理研究相结合，以更好地了解小麦籽粒中元素分布和储存的机制。这些研究将为提高营养价值和农艺措施提供基础知识。
The present study focused on the elemental distribution in the developing wheat grain by using the laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging technique. Our studies show that the embryo accumulated high concentrations of nutrient elements, such as Fe, K, Cu, and Zn, while Ca was accumulated in the bran of the wheat grain which might be attributed to its function of structural maintenance. In the endosperm the majority of the nutrients were located in the aleurone layer. Within the grain, the embryo could be considered as a nutrient pool for macro- and micro-elements essential for the development of the seedling. Elemental images showed that considerable amounts of nutrients were stored in the scutellum of the embryo, which might be related to the high gene expression of element transporters in the scutellum. Root primordia and leaf primordia were enriched in particular elements, such as Mn and Zn respectively. In total 34 cross sections were analyzed and used for generation of a sequence of elemental distribution images to demonstrate elemental changes along the perpendicular axis of the wheat grain embryo. Further development of three-dimensional modeling will be combined with physiological studies to better understand the mechanisms of elemental distribution and storage in the wheat grain. These studies will provide fundamental knowledge on improving the nutritional value and agronomic practices.