Comparative study of ZnO and TiO2 nanoparticles: physicochemical characterisation and toxicological effects on human colon carcinoma cells
Comparative study of ZnO and TiO2 nanoparticles: physicochemical characterisation and toxicological effects on human colon carcinoma cells
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DOI：
10.3109/17435390.2012.741724
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发表时间：
2013-12-01
期刊：
影响因子：
5
通讯作者：
De Berardis, Barbara
中科院分区：
文献类型：
--
作者：
De Angelis, Isabella;Barone, Flavia;De Berardis, Barbara
尽管人类胃肠道暴露于纳米颗粒(NPs)，但关于NPs对肠道细胞毒性的数据相当少。本研究评价了氧化锌和二氧化钛纳米颗粒对Caco-2细胞的毒性作用。只有氧化锌纳米颗粒具有显著的细胞毒性，通过两种不同的检测方法进行评估。在培养液中加入胎牛血清可显著降低氧化锌纳米颗粒的毒性以及离子渗漏和NP-细胞间的相互作用。处理6h后，两种NPs均能增加细胞内的ROS含量。然而，只有氧化锌纳米粒在6小时和24小时后都会增加ROS和诱导IL-8的释放。实验数据表明，化学成分和可溶性在氧化锌纳米粒毒性中起主要作用。此外，我们的结果表明，氧化应激在氧化锌纳米粒细胞毒性诱导中起关键作用，在无血清介质中，氧化应激既与离子泄漏有关，也与细胞与纳米粒的相互作用有关。
Despite human gastrointestinal exposure to nanoparticles (NPs), data on NPs toxicity in intestinal cells are quite scanty. In this study we evaluated the toxicity induced by zinc oxide (ZnO) and titanium dioxide (TiO2) NPs on Caco-2 cells. Only ZnO NPs produced significant cytotoxicity, evaluated by two different assays. The presence of foetal calf serum in culture medium significantly reduced ZnO NPs toxicity as well as ion leakage and NP-cell interaction. The two NPs increased the intracellular amount of reactive oxygen species (ROS) after 6 h treatment. However, only ZnO NPs increased ROS and induced IL-8 release both after 6 and 24 h. Experimental data indicate a main role of chemical composition and solubility in ZnO NPs toxicity.Moreover our results suggest a key role of oxidative stress in ZnO NPs cytotoxicity induction related both to ion leakage and to cell interaction with NPs in serum-free medium.