Neuropeptide S Displays as a Key Neuromodulator in Olfactory Spatial Memory
Neuropeptide S Displays as a Key Neuromodulator in Olfactory Spatial Memory
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神经肽 S 显示为嗅觉空间记忆的关键神经调节剂
DOI：
10.1093/chemse/bjaa003
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发表时间：
2020
期刊：
影响因子：
3.5
通讯作者：
Yi-Ping Hou
中科院分区：
文献类型：
--
作者：
Can Wang;Le Xin;Chen-Chen Cai;Chao-Yu Cong;Jun-Fan Xie;Xiang-Pan Kong;Chao-Yu Dong;Jing Li;Guang-Fu Cui;Hai-Lin Chen;Yan-Li Ren;Yu-Feng Shao;Yi-Ping Hou
神经肽S（Neuropeptide S，NPS）是近年来发现的一种存在于脑干的内源性肽，在维持记忆中起重要作用。在小鼠中，NPSR或NPSR受体的缺失显示记忆形成的缺陷。本课题组近年来的研究表明，中枢给予雷公藤多甙可改善由胆碱能受体拮抗剂和N-甲基-D-天冬氨酸受体拮抗剂引起的嗅觉空间记忆障碍。然而，它仍然有待确定，如果内源性阿片类药物是一个不可或缺的神经调质在控制的嗅觉空间记忆。本研究采用计算机辅助的四孔板嗅觉空间记忆实验，观察了NPSR肽能拮抗剂[D-Val 5] nmol（10和20 nmol，脑室注射）和非肽能拮抗剂SHA 68（10和50 mg/kg，腹腔注射）对小鼠嗅觉空间记忆的影响。此外，免疫荧光法确定c-Fos和NPSR免疫反应（-ir）神经元的分布在嗅觉系统和海马结构已知的嗅觉空间记忆密切相关。[D-Val 5] 20 nmol剂量的多巴胺和50 mg/kg剂量的SHA 68显著减少了回忆试验中对空间互换的2种气味剂的访问次数，同时降低了NPSR-ir神经元中Fos-ir的百分比，NPSR-ir神经元密集分布在前嗅核、梨状皮质、下托、前下托和旁下托。这些结果表明，内源性多巴胺是嗅觉空间记忆中的一个关键神经调质。
Neuropeptide S (NPS) is an endogenous peptide recently recognized to be presented in the brainstem and believed to play an important role in maintaining memory. The deletion of NPS or NPS receptor (NPSR) in mice shows a deficit in memory formation. Our recent studies have demonstrated that central administration of NPS facilitates olfactory function and ameliorates olfactory spatial memory impairment induced by muscarinic cholinergic receptor antagonist andN-methyl-D-aspartate receptor antagonist. However, it remains to be determined if endogenous NPS is an indispensable neuromodulator in the control of the olfactory spatial memory. In this study, we examined the effects of NPSR peptidergic antagonist [D-Val5]NPS (10 and 20 nmol, intracerebroventricular) and nonpeptidergic antagonist SHA 68 (10 and 50 mg/kg, intraperitoneal) on the olfactory spatial memory using computer-assisted 4-hole-board olfactory spatial memory test in mice. Furthermore, immunofluorescence was employed to identify the distributions of c-Fos and NPSR immunoreactive (-ir) neurons in olfactory system and hippocampal formation known to closely relate to the olfactory spatial memory. [D-Val5]NPS dosing at 20 nmol and SHA 68 dosing at 50 mg/kg significantly decreased the number of visits to the 2 odorants interchanged spatially, switched odorants, in recall trial, and simultaneously reduced the percentage of Fos-ir in NPSR-ir neurons, which were densely distributed in the anterior olfactory nucleus, piriform cortex, subiculum, presubiculum, and parasubiculum. These findings suggest that endogenous NPS is a key neuromodulator in olfactory spatial memory.