Alarm pheromone increases defensive and risk assessment behaviors in male rats
Alarm pheromone increases defensive and risk assessment behaviors in male rats
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DOI：
10.1016/j.physbeh.2005.11.003
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发表时间：
2006-02-28
影响因子：
2.9
通讯作者：
Mori, Y
中科院分区：
文献类型：
--
作者：
Kiyokawa, Y;Shimozuru, M;Mori, Y
此前，我们报道了雄性大鼠肛周区域释放的警报信息素会加重应激诱导的体温过高，并增加信息素受体大鼠犁鼻通路和应激相关核团中 Fos 的表达。然而，这种信息素在行为改变方面的报警特性仍不清楚。我们最近发现这种警报信息素可能被困在水中。基于这一发现，我们开发了一种实验范例来评估警报信息素对接收者行为的影响。使雄性Wistar大鼠适应开阔场地5分钟，将两张用750μl含信息素水或载体水浸泡过的滤纸贴在墙上。然后，将一个小“隐藏箱”放置在场地的一个角落，并记录受试大鼠在随后 10 分钟内的行为反应。与车辆对照组相比，暴露于警报信息素显着增加了防御和风险评估行为，并减少了探索和梳理行为，表明信息素的警报属性。此外，与之前的结果比较表明，雄性大鼠肛周区域释放的警报信息素会增加接受者的焦虑，而不是引起刻板的自主反应。 (c) 4 2005 Elsevier Inc. 保留所有权利。
Previously, we reported that alarm pheromone released from the perianal region of male rats aggravated stress-induced hyperthermia and increased Fos expression in the vomeronasal pathway and stress-related nuclei in pheromone-recipient rats. However, the alarm property of this pheromone in terms of behavior modification is still unclear. We recently found that this alarm pheromone could be trapped in water. Based on this finding, we developed an experimental paradigm to assess the effect of alarm pheromone on recipient behavior. Male Wistar rats were acclimatized for 5 min to an open field, where two pieces of filter paper soaked with 750 mu l of either pheromone-containing water or vehicle water were attached to the wall. Then, a small "hiding box" was placed in one corner of the field and the behavioral responses of the subject rat were recorded for 10 subsequent minutes. Exposure to alarm pheromone significantly increased defensive and risk assessment behaviors and decreased exploratory and grooming behaviors compared to the vehicle control group, indicating the alarm property of the pheromone. In addition, the comparison with previous results suggests that the alarm pheromone released from the perianal region of the male rat increases anxiety in recipients, rather than evoking a stereotyped autonomic response. (c) 4 2005 Elsevier Inc. All rights reserved.