Relationship between periodontitis-associated subgingival microbiota and clinical inflammation by 16S pyrosequencing
Relationship between periodontitis-associated subgingival microbiota and clinical inflammation by 16S pyrosequencing
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DOI：
10.1111/jcpe.12470
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发表时间：
2015-12-01
影响因子：
6.7
通讯作者：
Tomas, I.
中科院分区：
文献类型：
--
作者：
Camelo-Castillo, A.;Novoa, L.;Tomas, I.
目的：分析慢性牙周炎相关龈下菌群与临床炎症的关系。材料与方法：60例泛发性慢性牙周炎患者参加本研究。根据探诊出血（BOP）评分将患者分为两组：BOP-1组（平均评分50%）。结果：在所有分析的龈下标本中，共鉴定出102个细菌属和203个种（来自41个感兴趣的属）。稀疏曲线显示与BOP-1组相比，BOP-2组样品中的细菌种类数量更多。BOP-1组的厌氧球杆菌丰度显著高于其它组（特别是厌氧球杆菌A. geminatus）、Capnocytophaga（特别是C. gingivalis）、TM 7和韦荣氏球菌（Veillonella）。BOP-2的脱硫泡属丰度百分比显著高于其它两个种群（特别是D. propionicus）、真杆菌属（尤其是E. saphenum）、白链霉菌（Filifactoralocis）、星座链球菌（Streptococcusconstellatus）、坦纳氏菌（Tannerella）（尤其是坦纳氏菌（T. saphenum））、白链霉菌（Filifactoralocis）、星座链球菌（Streptococcusconstellatus）、坦纳氏菌（T. saphenum）等。结论：16 S焦磷酸测序揭示，在牙周炎部位增加的炎症与更多样化的龈下微生物群和细菌组成的特定变化相关，涉及“已建立的”牙周病原体、共生体和新的低丰度致病菌。
Aim: To analyse the relationship between the chronic periodontitis-associated subgingival microbiota and clinical inflammation.Material and Methods: Sixty subjects with generalized chronic periodontitis participated in this study. Patients were divided into two groups according to their bleeding on probing (BOP) scores: BOP-1 group (mean scores 50%). Subgingival bacterial samples from periodontal patients were studied by pyrosequencing PCR products of the 16S rRNA gene and by real-time PCR.Results: In all the analysed subgingival samples, 102 bacterial genera and 203 species (from 41 genera of interest) were identified. Rarefaction curves showed a greater number of bacterial species in samples from BOP-2 group compared to BOP-1 group. The BOP-1 group had significantly higher abundance percentages of Anaeroglobus (especifically, A. geminatus), Capnocytophaga (especifically C. gingivalis), TM7 and Veillonella. The BOP-2 had significantly higher abundance percentages of Desulfobulbus (especially D. propionicus), Eubacterium (especially E. saphenum), Filifactor alocis, Streptococcus constellatus, Tannerella (especially, T. forsythia) and Treponema.Conclusion: 16S pyrosequencing revealed that increased inflammation, at sites with periodontitis, is associated with a more diverse subgingival microbiota and specific changes in the bacterial composition, involving "established" periopathogens, symbionts and novel low-abundance pathobionts.