Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana
Identification of a receptor-like protein kinase gene rapidly induced by abscisic acid, dehydration, high salt, and cold treatments in Arabidopsis thaliana
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DOI：
10.1104/pp.113.4.1203
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发表时间：
1997-04-01
期刊：
影响因子：
7.4
通讯作者：
Nam, HG
中科院分区：
文献类型：
--
作者：
Hong, SW;Jon, JH;Nam, HG
从拟南芥中克隆了一个受体样蛋白激酶基因（RPK 1）的cDNA克隆。该克隆长1952 bp，具有1623 bp的开放阅读框，编码540个氨基酸的肽。推导的肽（RPK 1）包含四个独特的结构域的特征受体激酶：（a）一个假定的氨基末端信号序列结构域;（B）一个域与五个细胞外富含亮氨酸的重复序列;（c）跨膜结构域;和（d）胞质蛋白激酶结构域，包含所有的11个亚结构域保守的蛋白激酶。RPK 1基因在花、茎、叶和根中表达。在用脱落酸（阿坝）处理后1小时内诱导RPK 1基因的表达。该基因也被几种环境胁迫如脱水、高盐和低温快速诱导，表明该基因参与了一般的胁迫反应。脱水诱导的表达在阿坝-1，abi 1 -1，abi 2 -1和abi 3 -1突变体中没有受损，这表明RPK 1基因的脱水诱导的表达是不依赖于ABA的。讨论了该基因在阿坝信号转导途径和环境胁迫中的可能作用。
A cDNA clone for a receptor-like protein kinase gene (RPK1) was isolated from Arabidopsis thaliana. The clone is 1952 bp long with 1623 bp of an open reading frame encoding a peptide of 540 amino acids. The deduced peptide (RPK1) contains four distinctive domains characteristic of receptor kinases: (a) a putative aminoterminal signal sequence domain; (b) a domain with five extracellular leucine-rich repeat sequences; (c) a membrane-spanning domain; and (d) a cytoplasmic protein kinase domain that contains all of the 11 subdomains conserved among protein kinases. The RPK1 gene is expressed in flowers, stems, leaves, and roots. Expression of the RPK1 gene is induced within 1 h after treatment with abscisic acid (ABA). The gene is also rapidly induced by several environmental stresses such as dehydration, high salt, and low temperature, suggesting that the gene is involved in a general stress response. The dehydration-induced expression is not impaired in aba-1, abi1-1, abi2-1, and abi3-1 mutants, suggesting that the dehydration-induced expression of the RPK1 gene is ABA-independent. A possible role of this gene in the signal transduction pathway of ABA and the environmental stresses is discussed.