Characterization of a novel influenza hemagglutinin, H15: Criteria for determination of influenza a subtypes
Characterization of a novel influenza hemagglutinin, H15: Criteria for determination of influenza a subtypes
复制标题
DOI：
10.1006/viro.1996.0145
复制
发表时间：
1996-03-15
期刊：
影响因子：
3.7
通讯作者：
Webster, RG
中科院分区：
文献类型：
--
作者：
Rohm, C;Zhou, NA;Webster, RG
从澳大利亚的一只鸭和一只滨鸟中分离到两种具有新型血凝素（HA）的病毒，A/duck/Australia/341/83和A/shearwater/West Australia/2576/79。血凝抑制和双重免疫扩散试验未能显示与任何已知亚型（H1至H14）的交叉反应性。因此，我们建议这些病毒构成一个新的HA亚型，H15。HA基因序列分析证实了血清学结果。当在氨基酸水平上比较时，H15亚型的HA 1区与其它亚型的HA 1区相差30%或更多，这种程度的异质性在其它亚型的HA基因中也被发现。因此，我们建议在确定甲型流感病毒HA亚型时，应评估氨基酸序列数据。序列比较和系统发育分析表明，HA亚型H15与H7的亲缘关系最近。与H7 HA相比，H15在位于该分子的球状头部中的位置253处的HA 1内获得了30个核苷酸的插入。这一发现表明，RNA重组虽然在自然界中很罕见，但可能在流感病毒的进化中发挥重要作用。(C)出版社：Academic Press，Inc.
Two viruses with a novel hemagglutinin (HA), A/duck/Australia/341/83 and A/shearwater/West Australia/2576/79, have been isolated from a duck and a shorebird in Australia. Hemagglutination inhibition and double immunodiffusion assays failed to reveal cross-reactivity with any of the known subtypes (H1 to H14). We therefore propose that these viruses constitute a new HA subtype, H15. Sequence analysis of the HA genes confirmed the serologic findings. When compared at the amino acid level, the HA1 region of the H15 subtype differs from those of the other subtypes by 30% and more, This degree of heterogeneity is also found among HA genes of other subtypes. Thus we propose that amino acid sequence data should be evaluated when determining the HA subtypes of influenza A viruses. Sequence comparison and phylogenetic analysis suggested that the HA subtype H15 is most closely related to the H7. Compared to the H7 HA, the H15 acquired a 30-nucleotide insertion within HA1 at position 253 which is located in the globular head of the molecule. This finding suggests that RNA recombination, although a rare event in nature, may play an important role in the evolution of influenza viruses. (C) 1996 Academic Press, Inc.