Psychometric assessment of the Rat Grimace Scale and development of an analgesic intervention score.
Psychometric assessment of the Rat Grimace Scale and development of an analgesic intervention score.
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DOI：
10.1371/journal.pone.0097882
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pang DS
中科院分区：
文献类型：
--
作者：
Oliver V;De Rantere D;Ritchie R;Chisholm J;Hecker KG;Pang DS
我们有限的能力来评估自发疼痛的啮齿动物模型的痛苦的人类条件可能与翻译失败有希望的镇痛化合物在临床应用。如果自发性疼痛行为的测量可以用来产生镇痛干预评分，它们的使用可以扩大到指导镇痛药的使用，作为监管机构和道德和福利义务的强制要求。自发性疼痛的一种测量方法，老鼠鬼脸量表（RGS），最近被描述并显示出可靠性。然而，测量分数的可靠性是特定于上下文和内容的，并且需要进一步的测试来评估对异质设置（不同的模型、评分者、环境）的翻译。本研究的目的是在异质环境下使用大鼠鬼脸量表进行可靠性测试，并为其使用生成镇痛干预评分。在一项随机、盲法研究中，16只成年雌性大鼠围手术期接受三种镇痛治疗中的一种（皮下注射0.05 mg/kg丁丙诺啡，皮下注射1 mg/kg美洛昔康，口服0.2 mg/kg丁丙诺啡软膏）（遥测单元植入手术）。对大鼠进行术前、术后1-6小时和术后12小时的录像和图像采集，由3名盲法评分者和5名专家根据“疼痛/无疼痛”评估进行独立评分。评分用于计算分级间和分级内的信度，采用分级内相关系数，并通过受试者工作特征曲线分析生成镇痛干预评分。RGS评分显示出非常好的评分间和评分内信度（分别为0.85 [0.78-0.90 95% CI]和0.83[0.76-0.89]）。镇痛干预阈值大于0.67。这些数据表明，RGS是一种有用的工具，可以成功地应用于异质环境，并具有指导镇痛干预的潜力。
Our limited ability to assess spontaneous pain in rodent models of painful human conditions may be associated with a translational failure of promising analgesic compounds in to clinical use. If measurement of spontaneous pain behaviours can be used to generate an analgesic intervention score their use could expand to guide the use of analgesics, as mandated by regulatory bodies and ethical and welfare obligations. One such measure of spontaneous pain, the Rat Grimace Scale (RGS), has recently been described and shown to exhibit reliability. However, reliability of measurement scores is context and content specific, and further testing required to assess translation to a heterogenous setting (different model, raters, environment). The objectives of this study were to perform reliability testing with the Rat Grimace Scale in a heterogenous setting and generate an analgesic intervention score for its use. In a randomised, blinded study, sixteen adult female rats received one of three analgesia treatments (0.05 mg/kg buprenorphine subcutaneously, 1 mg/kg meloxicam subcutaneously, 0.2 mg/kg oral buprenorphine in jelly) peri-operatively (telemetry unit implantation surgery). Rats were video-recorded (before, 1–6 and 12 hours post-operatively) and images collected for independent scoring by three blinded raters using the RGS, and five experts based on “pain/no pain” assessment. Scores were used to calculate inter- and intra-rater reliability with an intraclass correlation coefficient and generate an analgesic intervention score with receiver operating characteristic curve analysis. The RGS scores showed very good inter- and intra-rater reliability (0.85 [0.78–0.90 95% CI] and 0.83 [0.76–0.89], respectively). An analgesic intervention threshold of greater than 0.67 was determined. These data demonstrate that the RGS is a useful tool which can be successfully employed in a heterogenous setting, and has the potential to guide analgesic intervention.