Neural crest induction by Xwnt7B in Xenopus
Neural crest induction by Xwnt7B in Xenopus
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DOI：
10.1006/dbio.1997.8820
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发表时间：
1998-02-01
影响因子：
2.7
通讯作者：
Hemmati-Brivanlou, A
中科院分区：
文献类型：
--
作者：
Chang, CB;Hemmati-Brivanlou, A
在早期发育期间，神经诱导后不久就会出现神经模式。在非洲爪哇，在开放的神经板阶段，一些尾化因素将前神经转化为后神经组织，而其他因素负责建立内侧极性，在神经管关闭后成为背腹(D-V)轴。许多Wnt配体在神经管中表达在不同的前后(A-P)和D-V结构域，暗示了在神经模式中的功能。在此，我们报道了非洲爪哇Wnt7b全长基因的克隆。Xwnt7B在与神经诱导剂noggin共注射的外胚层外植体和解离神经化的外胚层细胞中诱导神经峰标记Xslug和Xtwist。在体内，Xwnt7B在动物体内注射时扩大了Xtwist的表达结构域。我们的结果表明，WNT成员参与了神经管的背腹模式。(C)1998年学术出版社。
Neural patterning occurs soon after neural induction during early development. In Xenopus, several caudalizing factors transform anterior neural to posterior neural tissue at the open neural plate stages, while other factors are responsible for setting up mediolateral polarity which becomes the dorsoventral (D-V) axis after neural tube closure. Many Wnt ligands are expressed in the neural tube in distinct anteroposterior (A-P) and D-V domains, implying a function in neural patterning. Here we report the cloning of a full-length Xenopus Wnt7B gene. Xwnt7B induces neural crest markers Xslug and Xtwist in ectodermal explants coinjected with neural inducer noggin and in ectodermal cells neuralized by dissociation. In vivo, Xwnt7B expands the Xtwist expression domain when injected in the animal pole. Our results suggest that Wnt members are involved in dorsoventral patterning of the neural tube. (C) 1998 Academic Press.