Nanoshuttles propelled by motor proteins sequentially assemble molecular cargo in a microfluidic device.
Nanoshuttles propelled by motor proteins sequentially assemble molecular cargo in a microfluidic device.
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由马达蛋白推动的纳米梭在微流体装置中顺序组装分子货物。
作者：
D. Steuerwald;Susanna M Früh;Rudolf Griss;R. Lovchik;V. Vogel
由分子马达驱动蛋白提供动力的纳米梭具有从溶液中捕获和浓缩稀有分子以及以高通量方式运输、分选和组装它们的潜力。一个长期的目标是实现一条以纳米穿梭机为主力的分子组装线。利用它们来实现这一目的可能会让社区设计新的材料和纳米器件。实现这一目标的核心里程碑是将纳米穿梭机暴露于一系列不同的分子或构建块，并按顺序加载它们以构建分层结构、大分子或材料。在这里，我们通过利用两种迄今为止主要互补的技术的协同作用来解决这一挑战，纳米梭介导的主动运输和压力驱动的被动运输，集成到单个微流体装置中以展示分子组装线的实现。因此，多步骤协议可以被简化为高度并行和自主工作的芯片实验室：在每个反应室中，分析物或构建块从溶液中捕获，然后通过纳米梭穿过下一个室中的流体流动边界进行运输。因此，货物可以在一个程序中进行组装、修改、分析和最终卸载，操作人员只需一个步骤。
Nanoshuttles powered by the molecular motor kinesin have the potential to capture and concentrate rare molecules from solution as well as to transport, sort and assemble them in a high-throughput manner. One long-thought-of goal has been the realisation of a molecular assembly line with nanoshuttles as workhorses. To harness them for this purpose might allow the community to engineer novel materials and nanodevices. The central milestone towards this goal is to expose nanoshuttles to a series of different molecules or building blocks and load them sequentially to build hierarchical structures, macromolecules or materials. Here, we addressed this challenge by exploiting the synergy of two so far mostly complementary techniques, nanoshuttle-mediated active transport and pressure-driven passive transport, integrated into a single microfluidic device to demonstrate the realisation of a molecular assembly line. Multiple step protocols can thus be miniaturised to a highly parallelised and autonomous working lab-on-a-chip: in each reaction chamber, analytes or building blocks are captured from solution and are then transported by nanoshuttles across fluid flow boundaries in the next chamber. Cargo can thus be assembled, modified, analysed and eventually unloaded in a procedure that requires only one step by its operator.