Imaging cell morphology and physiology using X-rays.
Imaging cell morphology and physiology using X-rays.
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DOI：
10.1042/bst20180036
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发表时间：
2019-04
影响因子：
3.9
通讯作者：
V. Weinhardt;Jian-Hua Chen;A. Ekman;G. McDermott;M. Le Gros;C. Larabell
中科院分区：
文献类型：
--
作者：
V. Weinhardt;Jian-Hua Chen;A. Ekman;G. McDermott;M. Le Gros;C. Larabell
形态测量，如量化细胞形状，表征亚细胞组织和探测细胞-细胞相互作用，是细胞生物学和临床医学的基础。直到最近，细胞形态测量数据的主要来源一直是基于光和电子的显微镜图像。然而，许多技术进步已经推动X射线显微镜成为另一种高质量形态测量信息的来源。在这里，我们回顾了X射线显微镜作为一种定量生物成像方式的地位。我们还描述了X射线显微镜数据与来自其他方式的信息相结合，以生成生物系统的多色视图。例如，来自荧光显微镜或光谱显微镜的分子定位数据与来自X射线断层扫描的结构信息的融合。来自相同样品的数据的这种组合生成比通过单一显微镜方法可以获得的系统的更完整的图片。这种多模态组合通过结合生理学和形态学数据来创建更准确地反映生命复杂性的模型，极大地增强了我们对生物学的理解。
Morphometric measurements, such as quantifying cell shape, characterizing sub-cellular organization, and probing cell-cell interactions, are fundamental in cell biology and clinical medicine. Until quite recently, the main source of morphometric data on cells has been light- and electron-based microscope images. However, many technological advances have propelled X-ray microscopy into becoming another source of high-quality morphometric information. Here, we review the status of X-ray microscopy as a quantitative biological imaging modality. We also describe the combination of X-ray microscopy data with information from other modalities to generate polychromatic views of biological systems. For example, the amalgamation of molecular localization data, from fluorescence microscopy or spectromicroscopy, with structural information from X-ray tomography. This combination of data from the same specimen generates a more complete picture of the system than that can be obtained by a single microscopy method. Such multimodal combinations greatly enhance our understanding of biology by combining physiological and morphological data to create models that more accurately reflect the complexities of life.