Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions
Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions
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DOI：
10.1016/j.gene.2008.05.002
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发表时间：
2008-08-01
期刊：
影响因子：
3.5
通讯作者：
Goto, Shin G.
中科院分区：
文献类型：
--
作者：
Ikeno, Tornoko;Numata, Hideharu;Goto, Shin G.
虽然昆虫生物钟的分子机制和多样性在全变态昆虫中已经得到了很好的研究，但半变态昆虫却很少受到关注。在本研究中，我们分离的昼夜节律钟基因，周期（每），周期（cyc），vrille（vri），并从豆错误Riptortus pedestris的隐花色素（cry-m）。这是半变态昆虫中vri和cry-m的首次报道。这些基因与其它昆虫的同源基因具有很高的相似性。cry-m在R. pedestris指出，半变态昆虫的时钟机制类似于包括帝王蝶Danaus plexippus和蜜蜂Apis mellifera在内的具有半变态的昆虫的时钟机制，而不类似于缺乏半变态的昆虫，如果蝇。实时荧光定量PCR结果显示，这些生物钟基因的mRNA在第9天的红细胞头部表现出极弱的昼夜节律振荡。pedestris，和他们的表达水平在长和短日照条件下几乎相同。此外，在两种光周期条件下，从0到15天，每mRNA的表达水平几乎是稳定的。长日照和短日照条件下mRNA水平的差异似乎太小，无法清楚地区分光周期条件。这些结果表明，昼夜节律钟基因的转录调控在红蜘蛛滞育程序中不起重要作用。pedestris。(C)2008 Elsevier B. V.保留所有权利。
Although the molecular mechanisms and the diversity of insect circadian clocks have been well investigated in holometabolous insects, hemimetabolous insects have received little attention. In the present study, we isolated the circadian clock genes, period (per), cycle (cyc), vrille (vri), and mammalian-type cryptochrome (cry-m) from the bean bug Riptortus pedestris. This is the first report of vri and cry-m in hemimetabolous insects. All of the genes showed high similarities to respective homologous genes in other insects. The discovery of cry-m in R. pedestris indicates that I he clockwork of hemimetabolous insects is similar to that in insects having CRY-m, including the monarch butterfly Danaus plexippus and the honey bee Apis mellifera, and not to insects lacking it, such as Drosophila melanogaster. Real-time PCR showed that mRNAs of these circadian clock genes exhibited extremely weak diel oscillations at day 9 in the head of R. pedestris, and their expression levels under long- and short-day conditions were nearly identical. In addition, expression levels of per mRNA were almost stable from days 0 to 15 tinder both photoperiodic conditions. The difference between long-day and short-day conditions in the mRNA level seems too small to distinguish photoperiodic conditions clearly. These results suggest that transcriptional regulations of circadian clock genes would not play an important role in the diapause programming in R. pedestris. (C) 2008 Elsevier B.V. All rights reserved.