Partitioning of 14C-oxalate excretion in rats during a persistent oxalate challenge.
Partitioning of 14C-oxalate excretion in rats during a persistent oxalate challenge.
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持续草酸盐挑战期间大鼠 14C-草酸盐排泄的分配。
DOI：
10.1007/s00240-008-0155-3
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发表时间：
2008
影响因子：
--
通讯作者：
Traverso,EdwardJ
中科院分区：
文献类型：
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作者：
Marengo,SusanRuth;Zhang,Ailin;Traverso,EdwardJ
这项研究是为了解决已发表的人类和大鼠之间草酸排泄的差异，并表征大鼠在持续严重高尿酸血症期间的草酸盐分配，例如在许多肥胖患者中观察到的。皮下植入分配360 μmole/天KOx + 3.9 ± 0.14 μCi/天14 C-草酸盐的渗透微型泵。收集所有排泄物。在第13天处死大鼠，解剖尸体，研磨，溶解在HCl中，并进行闪烁计数，回收了92.1 ± 3.9%的总草酸盐。这在皮肤复合物（38.2 ± 7.7%）、尸体复合物（24.5 ± 5.9%）和排泄物（29.5 ± 1.9%）中分配。草酸盐在皮肤和尸体复合物中的分布使我们推断只有29.5 ± 1.9%的给药草酸盐进入循环。在循环的草酸盐中，98.4 ± 0.4%被排泄（总尿液78.9 ± 1.7%;原始粪便21.0 ± 1.7%）。因此，大部分进入循环的草酸盐在大鼠中迅速排出，就像在人类中一样。因此，即使在一个大的，持续的草酸挑战，很少草酸积累在内部器官，肌肉和骨骼。然而，与人类不同的是，大鼠在粪便中排出了大量草酸盐。
This study was done to resolve published discrepancies in oxalate excretion between humans and rats and to characterize oxalate partitioning in rats during persistent severe hyperoxaluria, such as that seen in many bariatric patients. Osmotic minipumps dispensing 360 μmole/day KOx + 3.9 ± 0.14 μCi/day14C-oxalate were implanted subcutaneously. All excreta were collected. Rats were killed on day 13 and carcasses were dissected, ground, dissolved in HCl, and subjected to scintillation counting, and 92.1 ± 3.9% of total oxalate administered was recovered. This was partitioned among the skin complex (38.2 ± 7.7%), carcass complex (24.5 ± 5.9%), and excreta (29.5 ± 1.9%). The distribution of oxalate in the skin and carcass complexes led us to infer that only 29.5 ± 1.9% of the administered oxalate entered the circulation. Of the circulated oxalate, 98.4 ± 0.4% was excreted (total urine 78.9 ± 1.7%; raw feces 21.0 ± 1.7%). Thus, most oxalate that does enter the circulation is promptly excreted in rats, as in humans. Consequently, even after a large, persistent oxalate challenge, very little oxalate had accumulated in the internal organs, muscle, and skeleton. Unlike humans, however, rats excrete a significant fraction of oxalate in the feces.