EXPRESSION OF M-CADHERIN, A MEMBER OF THE CADHERIN MULTIGENE FAMILY, CORRELATES WITH DIFFERENTIATION OF SKELETAL-MUSCLE CELLS
EXPRESSION OF M-CADHERIN, A MEMBER OF THE CADHERIN MULTIGENE FAMILY, CORRELATES WITH DIFFERENTIATION OF SKELETAL-MUSCLE CELLS
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DOI：
10.1073/pnas.88.18.8024
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
STARZINSKIPOWITZ, A
中科院分区：
文献类型：
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作者：
DONALIES, M;CRAMER, M;STARZINSKIPOWITZ, A
钙粘蛋白是跨膜糖蛋白的多基因家族，介导Ca2+依赖性细胞间粘附。它们被认为是控制包括肌发生在内的形态发生过程所必需的。在这里，我们报告了从分化的肌肉细胞中鉴定和表征cadherin家族的另一个成员M-cadherin （M代表肌肉）的cDNA。最长的开放阅读框或分离的cdna几乎包含成熟的m -钙粘蛋白的整个编码区，与已知的钙粘蛋白序列同源。m -钙粘蛋白mRNA在成肌细胞中低水平存在，在肌管形成细胞中上调。在小鼠L细胞（成纤维细胞）中，m -钙粘蛋白mRNA无法检测到。这种表达模式表明m -钙粘蛋白是肌生成程序的一部分，并可能触发末梢肌肉分化。
Cadherins, a multigene family of transmembrane glycoproteins, mediate Ca2+-dependent intercellular adhesion. They are thought to be essential for the control of morphogenetic processes, including myogenesis. Here we report the identification and characterization of the cDNA of another member of the cadherin family, M-cadherin (M for muscle), from differentiating muscle cells. The longest open reading frame or the cDNAs isolated contains almost the entire coding region of the mature M-cadherin as determined by sequence homology to the known cadherins. M-cadherin mRNA is present at low levels in myoblasts and is upregulated in myotube-forming cells. In mouse L cells (fibroblasts), M-cadherin mRNA is undetectable. This expression pattern indicates that M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.