The first complete mitochondrial genome sequences of Amblypygi (Chelicerata: Arachnida) reveal conservation of the ancestral arthropod gene order
The first complete mitochondrial genome sequences of Amblypygi (Chelicerata: Arachnida) reveal conservation of the ancestral arthropod gene order
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DOI：
10.1139/g09-023
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发表时间：
2009-05-01
期刊：
影响因子：
3.1
通讯作者：
Podsiadlowski, Lars
中科院分区：
文献类型：
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作者：
Fahrein, Kathrin;Masta, Susan E.;Podsiadlowski, Lars
鞭蛛（Amblypygi）是生活在亚热带和热带的陆栖螯肢类动物。在形态学和基于rRNA的系统发育分析中，Amblypygi与Uropygi（鞭蝎）和Araneae（蜘蛛）聚在一起形成Tetrapulmonata分类群，但这三个分类群的相互关系存在争议。线粒体基因组提供了一个额外的大数据集的系统发育信息（序列，基因顺序，RNA二级结构），但在蛛形纲动物，线粒体基因组数据缺失的一些主要目的。在一个正在进行的项目过程中，关于蛛形纲动物线粒体基因组学，我们提出了前两个完整的线粒体基因组Amblypygi。这两个基因组被发现是典型的环状双链DNA分子，所有37个基因通常存在于双侧线粒体基因组中。在这两个物种中，基因顺序是相同的，鲎polyphemus（剑水蚤），这是假设反映了假定的节肢动物地面模式。所有的tRNA基因序列都有可能折叠成后生动物线粒体tRNA的典型结构，除了tRNA-Ala，它在两个amblypygids中都缺乏D臂，这表明在amblypygid进化的早期失去了这一功能。系统发育分析表明，尾臀亚目是钝尾亚目的姐妹类群的支持率较低。
Amblypygi (whip spiders) are terrestrial chelicerates inhabiting the subtropics and tropics. In morphological and rRNA-based phylogenetic analyses, Amblypygi cluster with Uropygi (whip scorpions) and Araneae (spiders) to form the taxon Tetrapulmonata, but there is controversy regarding the interrelationship of these three taxa. Mitochondrial genomes provide an additional large data set of phylogenetic information (sequences, gene order, RNA secondary structure), but in arachnids, mitochondrial genome data are missing for some of the major orders. In the course of an ongoing project concerning arachnid mitochondrial genomics, we present the first two complete mitochondrial genomes from Amblypygi. Both genomes were found to be typical circular duplex DNA molecules with all 37 genes usually present in bilaterian mitochondrial genomes. In both species, gene order is identical to that of Limulus polyphemus (Xiphosura), which is assumed to reflect the putative arthropod ground pattern. All tRNA gene sequences have the potential to fold into structures that are typical of metazoan mitochondrial tRNAs, except for tRNA-Ala, which lacks the D arm in both amblypygids, suggesting the loss of this feature early in amblypygid evolution. Phylogenetic analysis resulted in weak support for Uropygi being the sister group of Amblypygi.