Basement membrane recovery process in rat soleus muscle after exercise-induced muscle injury
Basement membrane recovery process in rat soleus muscle after exercise-induced muscle injury
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DOI：
10.1080/03008207.2020.1791839
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发表时间：
2020-07-25
影响因子：
2.9
通讯作者：
Shigeyoshi, Yasufumi
中科院分区：
文献类型：
--
作者：
Kanazawa, Yuji;Nagano, Mamoru;Shigeyoshi, Yasufumi
目的IV型胶原是基底膜的一种成分，为肌肉纤维提供机械支持。此外，转录因子4(TCF4)在肌肉结缔组织成纤维细胞中高表达，并调节肌肉再生。然而，IV型胶原和TCF4(+)细胞在运动性肌肉损伤中的表达还不是很清楚。在此，我们研究了不同运动负荷所致肌肉损伤后恢复过程中IV型胶原和TCF4(+)细胞的表达和定位。材料与方法幼年Wistar大鼠在跑台上进行12次和18次下坡跑(DR)后，观察比目鱼肌的肌肉损伤情况。跑步结束后，允许大鼠恢复0.5d、2d、7d。结果糖尿病视网膜病变后损伤肌纤维中可见IV型胶原的异位定位，18例糖尿病视网膜病变后0.5d及12或18例糖尿病视网膜病变后2d，IV型胶原蛋白的数量较术前明显增多。恢复0.5d时，损伤肌纤维内及周围出现Dr.TCF4(+)细胞后，观察到BM断裂。18次DR后，TCF4(+)细胞的数量比12次DR后的多，持续时间更长。结论DR可导致骨髓破坏并伴有肌纤维损伤。BM的破坏可能伴随着肌肉的损伤，TCF4(+)细胞可能有助于肌肉纤维和BM的恢复。
Purpose Collagen IV is a component of the basement membrane (BM) that provides mechanical support for muscle fibers. In addition, transcription factor 4 (TCF4) is highly expressed in muscle connective tissue fibroblasts and regulates muscle regeneration. However, the expression of collagen IV and TCF4 (+) cells in response to exercise-induced muscle injury is not well known. Here, we investigated the expression and localization of collagen IV and TCF4 (+) cells during the recovery process after muscle injury induced by different exercise loads. Materials and Methods Muscle injury was observed in the soleus muscle of young Wistar rats after 12 or 18 sets-downhill running (DR) on a treadmill. After running, the rats were permitted to recover for a period of 0.5 days, 2 days, or 7 days. Results Ectopic localization of collagen IV in injured muscle fibers was observed after DR, and the number increased at 0.5 days after 18 sets DR and at 2 days after 12 or 18 sets DR as compared to the number observed at baseline. BM disruption was observed after DR. TCF4 (+) cells appeared in the inside and around injured muscle fibers at 0.5 day of recovery. After 18 sets DR, TCF4 (+) cells were more abundant for a longer period than that observed after 12 sets DR. Conclusions DR induces BM disruption accompanied by muscle fiber damage. It is possible that BM destruction may be accompanied by muscle damage and that TCF4 (+) cells contribute to muscle fiber and BM recovery.