Transmission of swine influenza virus to humans after exposure to experimentally infected pigs.
Transmission of swine influenza virus to humans after exposure to experimentally infected pigs.
复制标题
接触实验感染的猪后，猪流感病毒会传播给人类。
DOI：
10.1093/infdis/175.1.7
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Hinshaw,VS
中科院分区：
文献类型：
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作者：
Wentworth,DE;McGregor,MW;Macklin,MD;Neumann,V;Hinshaw,VS
2人在从实验感染A/Sw/IN/1726/88（Sw/IN）的猪采集鼻拭子后36小时出现流感症状。这些人的咽拭子在感染后8天检测流感病毒RNA呈阳性。半巢式聚合酶链反应（PCR）产物分析表明，分离物的血凝素（HA）片段的遗传相关的Sw/IN的HA。从一名39岁男性中回收了4株甲型流感病毒分离株（A/WI/4754/94、A/WI/4756/94、A/WI/4758/94、A/WI/4760/94），从一名31岁女性中回收了2株（A/WI/4755/94、A/WI/4757/94）。分离株的HA与用于感染猪的病毒在抗原性上无法区分。HA基因的序列分析表明，它们与实验中使用的病毒的HA具有99.7%的同一性。多片段逆转录-PCR证明所有片段均源自Sw/IN，表明猪H1N1病毒直接且容易地传播给人类，尽管这些实验使用了动物生物安全3级控制措施。
Two people developed symptoms of influenza 36 h after collecting nasal swabs from pigs experimentally infected with A/Sw/IN/1726/88 (Sw/IN). Pharyngeal swabs from these persons tested positive for influenza virus RNA 8 days after infection. Analysis of hemi-nested polymerase chain reaction (PCR) products indicated that the hemagglutinin (HA) segments of the isolates were genetically related to the HA of Sw/IN. Four influenza A virus isolates (A/WI/4754/94, A/WI/4756/94, A/WI/4758/94, A/WI/4760/94) were recovered from a 39-year-old man and 2 (A/WI/4755/94,A/WI/4757/94) from a 31-year-old woman. The HAs of the isolates were antigenically indistinguishable from the virus used to infect the pigs. Sequence analysis of the HA genes indicated they were 99.7% identical to the HA of the virus used in the experiment. Multisegment reverse transcription–PCR proved that all of the segments originated from Sw/IN, demonstrating that transmission of swine H1N1 viruses to humans occurs directly and readily, despite Animal Biosafety Level 3 containment practices used for these experiments.