Genetic transformation of Populus deltoides and Px euramericana clones using Agrobacterium tumefaciens
Genetic transformation of Populus deltoides and Px euramericana clones using Agrobacterium tumefaciens
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DOI：
10.1023/a:1005838032153
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发表时间：
1997-01-01
影响因子：
3
通讯作者：
Cella, R
中科院分区：
文献类型：
--
作者：
Confalonieri, M;Balestrazzi, A;Cella, R
通过接种实验评估了不同美洲杨（Neva、PE68-022 x P. nigra、71-060 x P. nigra）和美洲杨（PE68-022 x P. deltoides）对野生型根癌农杆菌菌株（A281和82.139）的敏感性，以及肿瘤形成频率的差异。 (0-48%)被发现。共培养实验表明，与解除武装的菌株相比，致癌二元农杆菌菌株具有较高的转化能力。使用致癌二元菌株，从所有测试的克隆中获得转基因愈伤组织。乙酰丁香酮的存在不影响解除武装菌株的转化频率。使用叶盘和含有野生型和解除武装菌株的细菌悬浮液进行联合接种实验。在这些条件下也没有注意到对转化效率的积极影响。 DNA分析证实了肿瘤和卡那霉素抗性愈伤组织的转化。
The susceptibility of different Populus euramericana (Neva, PE68-022 x P. nigra, 71-060 x P. nigra) and P. deltoides (PE68-022 x P. deltoides) crones to wild-type Agrobacterium tumefaciens strains (A281 and 82.139) was evaluated in an inoculation experiment, and differences in the frequency of tumor formation (0-48%) were found. Co-cultivation experiments demonstrated high transformation ability of oncogenic binary A. tumefaciens strains as compared to disarmed strains. Using oncogenic binary strains, transgenic calluses were obtained from all tested clones. The presence of acetosyringone did not influence the transformation frequency of the disarmed strains. Co-inoculation experiments were performed using leaf discs and a bacterial suspension containing both wild-type and disarmed strains. No positive effects on transformation efficiency were noticed in these conditions either. The transformation of tumors and kanamycin resistant calluses was confirmed by DNA analysis.