A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.
复制标题
含有遗传连锁的交替抗生素抗性元件的菌株集合，用于大肠杆菌的遗传图谱。
DOI：
10.1128/mr.53.1.1-24.1989
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Gross,CA
中科院分区：
文献类型：
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作者：
Singer,M;Baker,TA;Schnitzler,G;Deischel,SM;Goel,M;Dove,W;Jaacks,KJ;Grossman,AD;Erickson,JW;Gross,CA
我们收集了182个等基因菌株，这些菌株含有位于大肠杆菌染色体周围约1分钟间隔的遗传连锁抗生素耐药元件。在大多数位置，Tn10 （Tetr）和TN10kan （Kanr）元素都可用，因此收集集包含一组相互关联的交替抗生素耐药标记。每个插入的图谱位置已与大肠杆菌遗传图谱以及Kohara命令克隆库对齐。这些菌株被设计用于大肠杆菌的快速两步定位系统。第一步，通过Hfr定位，将一组含有Tn10或Tn10kan元件的Hfr菌株定位在距离其各自转移起点20分钟的染色体上，将突变定位在5- 15分钟的区域。在第二步中，突变通过P1转导定位到1分钟区域，收集等基因插入菌株作为供体。我们讨论了利用这些菌株来绘制并最终克隆大肠杆菌中的各种突变。
We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most positions both Tn10 (Tetr) and TN10kan (Kanr) elements are available, so that the collection contains a linked set of alternating antibiotic resistance markers. The map position of each insertion has been aligned to the E. coli genetic map as well as to the Kohara ordered clone bank. These strains are designed to be used in a rapid two-step mapping system in E. coli. In the first step, the mutation is localized to a 5- to 15-min region of the chromosome by Hfr mapping with a set of Hfr strains containing either Tn10 or Tn10kan elements located 20 min from their respective origins of transfer. In the second step, the mutation is localized to a 1-min region by P1 transduction, with a collection of isogenic insertion strains as donors. We discuss the uses of this collection of strains to map and eventually to clone a variety of mutations in E. coli.