Isolation decreases physical and motivational aspects of morphine withdrawal
Isolation decreases physical and motivational aspects of morphine withdrawal
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DOI：
10.1097/00008877-200505000-00001
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发表时间：
2005-05-01
影响因子：
1.6
通讯作者：
Mi単arro, J
中科院分区：
文献类型：
--
作者：
Broseta, I;Rodriguez-Arias, M;Mi単arro, J
环境操作，如动物的社会住房条件可能在表达对药物的反应的个体差异中发挥作用。本研究的目的是评估是否隔离和分组小鼠产生不同程度的吗啡依赖。采用两种不同的诱导方法使分离和分组的小鼠产生吗啡依赖性：快速或慢速方案，两者均达到相同的最大日剂量（1100 mg/kg）。采用改良的Gellert-Holtzman量表和条件性位置厌恶（CPA）程序评估纳洛酮诱导的吗啡戒断。隔离的动物表现出较少的迹象，身体依赖性比分组小鼠，只有那些接受每天两次吗啡剂量的Gellert-Holtzman量表上的分数显着高于对照组。同样，在CPA中，尽管所有吗啡处理的动物都产生了厌恶，但其强度仅显著高于接受两次每日剂量的分组动物中的对照组。用热板试验测量的镇痛反应表明，隔离的小鼠表现出更长的舔爪时间（即使没有给药），表明它们具有更高水平的内源性阿片类物质。可以说，隔离动物对吗啡的敏感性可能不如非隔离动物，因此可以耐受更大的剂量或需要更多的药物才能产生相同的效果。结果表明，阿片类药物反应的变异性可能受到环境操作的影响。
Environmental manipulations such as social housing conditions of animals may play a role in the expression of individual differences in response to drugs. This study aimed to evaluate whether isolated and grouped mice develop different degrees of morphine dependence. Isolated and grouped mice were rendered morphine dependent employing two different methods of induction: a fast or slow protocol, both reaching the same maximum daily dose (1100 mg/kg). Naloxone-induced morphine withdrawal was assessed using a modified Gellert-Holtzman scale and a conditioned place aversion (CPA) procedure. Isolated animals manifested fewer signs of physical dependence than grouped mice and only those receiving two daily morphine doses presented significantly higher scores on the Gellert-Holtzman scale than controls. Similarly, in CPA, although all morphine-treated animals developed aversion, its intensity was only significantly higher than in controls in grouped animals receiving two daily doses. Analgesic response, measured with the hot-plate test, showed that isolated mice presented longer latencies to lick their paws (even without drug administration), suggesting that they had a higher level of endogenous opiates. It can be argued that isolated animals may be less sensitive to morphine than the non-isolated and therefore tolerate greater quantities or require more drug to produce the same effects. The results suggest that variability in the response to opiates could be affected by environmental manipulations.