Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress
Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress
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DOI：
10.1104/pp.126.3.1196
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发表时间：
2001-07-01
期刊：
影响因子：
7.4
通讯作者：
Lamattina, L
中科院分区：
文献类型：
--
作者：
Mata, CG;Lamattina, L
一氧化氮（NO）是一种非常活跃的分子，参与许多不同的生物学途径，已证明其对氧化应激条件引起的损伤具有保护作用。本文研究了硝普钠（SNP）和S-亚硝基-N-乙酰青霉胺（S-nitroso-N-acetylpenicillamine，S-NNP）两种NO供体对小麦（Triticum aestivum）水分胁迫响应的影响。经过2和3小时的干旱，离体小麦叶片预处理150 μ M SNP保留高达15%以上的水比那些预处理水或NO2-/NO3-。干旱7 d后，SNP处理对小麦幼苗的保水性也有影响。这些结果与SNP处理的小麦离体叶片在相同分析时间内的蒸腾速率降低20%一致。在平行实验中，NO也能够诱导35%，30%，和65%的气孔关闭在三个不同的物种，紫露草属（单子叶植物）和两个双子叶植物，Salpichroa organifolia和蚕豆（蚕豆），分别。在SNP处理的紫露草属植物叶片中，气孔关闭与RWC增加10%相关。恢复期后，SNP处理的小麦叶片的离子渗漏（细胞损伤指标）比对照低25%。羧基-PTIO（2-（4-carboxyphenyl）-4，4，5，5-tetramethylimidazolin 1-oxyl-3-oxide）是一种特异的NO清除剂，通过恢复蒸腾速率、气孔开度和离子渗漏到未处理叶片的水平来恢复SNP的作用。Northern-blot分析表明，SNP处理的小麦叶片显示2倍积累的第三组晚期胚胎发生丰富的转录相对于对照叶片后2和4小时的干旱期。总之，这些结果表明，外源性应用NO供体可能赋予植物对严重干旱胁迫条件的耐受性增加。
Nitric oxide (NO) is a very active molecule involved in many and diverse biological pathways where it has proved to be protective against damages provoked by oxidative stress conditions. In this work, we studied the effect of two NO donors, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine SNP-treated on the response of wheat (Triticum aestivum) to water stress conditions. After 2 and 3 h of drought, detached wheat leaves pretreated with 150 muM SNP retained up to 15% more water than those pretreated with water or NO2-/NO3-. The effect of SNP treatment on water retention was also found in wheat seedlings after 7 d of drought. These results were consistent with a 20% decrease in the transpiration rate of SNP-treated detached wheat leaves for the same analyzed time. In parallel experiments, NO was also able to induce a 35%, 30%, and 65% of stomatal closure in three different species, Tradescantia sp. (monocotyledonous) and two dicotyledonous, Salpichroa organifolia and fava bean (Vicia faba), respectively. In SNP-treated leaves of Tradescantia sp. the stomatal closure was correlated with a 10% increase on RWC. Ion leakage, a cell injury index, was 25% lower in SNP-treated wheat leaves compared with control ones after the recovery period. Carboxy-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazolin 1-oxyl-3-oxide), a specific NO scavenger, reverted SNP action by restoring the transpiration rate, stomatal aperture, and the ion leakage to the level found in untreated leaves. Northern-blot analysis showed that SNP-treated wheat leaves display a 2-fold accumulation of a group three late embryogenesis abundant transcript with respect to control leaves both after 2 and 4 h of drought periods. All together, these results suggest that the exogenous application of NO donors might confer an increased tolerance to severe drought stress conditions in plants.