Dual tagging of GFP and Degron on endogenous COSA-1 in Caenorhabditis elegans as a crossover investigation tool.
Dual tagging of GFP and Degron on endogenous COSA-1 in Caenorhabditis elegans as a crossover investigation tool.
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DOI：
10.17912/micropub.biology.001087
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发表时间：
2024
影响因子：
--
通讯作者：
Wang, Bin
中科院分区：
文献类型：
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作者：
Odiba, Arome Solomon;Liao, Guiyan;Yuan, Haiyan;Fang, Wenxia;Wang, Bin
COSA-1是线虫精确减数分裂所必需的。对两个零突变体(COSA-1(Me13)和COSA-1(Tm3298))进行了重点研究。这些零突变体表现出严重的减数分裂缺陷，阻碍了对COSA-1功能微妙或动态性质的观察。为了克服这些限制，我们利用生长素诱导的Degron(AID)系统开发了一株可诱导降解COSA-1的线虫菌株。该毒株表现出正常的生育能力和COSA-1：：GFP焦点。生长素处理成功地耗尽COSA-1，导致子代存活率下降96%，并导致终变期卵母细胞中12条单价染色体。该菌株为研究COSA-1的动力学提供了有价值的工具。
COSA-1 is essential for accurate meiosis in C. elegans . Two null mutants ( cosa-1 ( me13 ) and cosa-1 ( tm3298 ) ) have been notably studied. These null mutants exhibit severe meiotic defects, hindering the observation of the subtle or dynamic nature of COSA-1 function. To overcome these limitations, we developed a C. elegans strain with inducible COSA-1 degradation using the Auxin-Inducible Degron (AID) system. This strain exhibits normal fertility and COSA-1::GFP foci. Auxin treatment successfully depletes COSA-1, resulting in a 96% decrease in progeny viability and 12 univalent chromosomes in diakinesis oocytes. This strain serves as a valuable tool for studying the dynamics of COSA-1.