Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs.
Mycobacterium tuberculosis whole cell lysate enhances proliferation of CD8 positive lymphocytes and nitric oxide secretion in the lungs of live porcine respiratory and reproductive syndrome virus vaccinated pigs.
复制标题
结核分枝杆菌全细胞裂解物可增强活猪呼吸与生殖综合征病毒疫苗接种猪肺部 CD8 阳性淋巴细胞的增殖和一氧化氮的分泌。
DOI：
10.1089/vim.2012.0065
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发表时间：
2013
期刊：
影响因子：
2.2
通讯作者：
Renukaradhya,GourapuraJ
中科院分区：
文献类型：
--
作者：
Manickam,Cordelia;Dwivedi,Varun;Miller,Jayla;Papenfuss,Tracey;Renukaradhya,GourapuraJ
猪呼吸与繁殖综合征（PRRS）是世界范围内猪的一种重要的经济疾病。目前使用的PRRSV疫苗提供不完全保护。最近，我们发现结核分枝杆菌全细胞裂解液（MtbWCL）作为修饰的PRRSV活疫苗（PRRS-MLV）的有效粘膜佐剂。在这项研究中，猪未接种或接种了PRRS-MLV加mbmwcl，鼻内接种，并用同源（株VR2332）或毒力强的异源（株MN184） PRRSV攻毒；随后，在攻毒后的三个时间点实施安乐死，以评估肺部免疫反应。肺切片显微镜检查显示，与未接种MN184疫苗的猪相比，接种疫苗的猪肺结构破坏减少，间质性肺炎减少。接种MN184后，肺和外周血单核细胞CD8+淋巴细胞增殖增加，肺匀浆中一氧化氮分泌增加。综上所述，mbmwcl对PRRS-MLV的佐剂作用导致肺部有利的抗prrsv免疫微环境，有助于更好地清除病毒。
Porcine respiratory and reproductive syndrome (PRRS) is an economically important disease of pigs worldwide. Currently used PRRSV vaccines provide incomplete protection. Recently, we identifiedMycobacterium tuberculosiswhole cell lysate (MtbWCL) as a potent mucosal adjuvant to modified live PRRSV vaccine (PRRS-MLV). In this study, pigs were unvaccinated or vaccinated with PRRS-MLV plusMtbWCL, intranasally, and challenged with either homologous (strain VR2332) or virulent heterologous (strain MN184) PRRSV; subsequently, euthanized at three time points post-challenge to evaluate lung immune responses. Microscopic examination of lung sections revealed reduced disruption of the lung architecture and less of interstitial pneumonia in vaccinated, compared to unvaccinated MN184 challenged pigs. The restimulated lung and peripheral blood mononuclear cells revealed increased proliferation of CD8+lymphocytes, and in the lung homogenate increased secretion of nitric oxide was detected in vaccinated MN184 challenged pigs. In summary, the adjuvant effects ofMtbWCL to PRRS-MLV resulted in favorable anti-PRRSV immune microenvironment in the lungs to help better viral clearance.