Evidence for the existence of ganglioside molecules on Pneumocystis carinii from human lungs
Evidence for the existence of ganglioside molecules on Pneumocystis carinii from human lungs
复制标题
人肺卡氏肺孢子虫上存在神经节苷脂分子的证据
作者：
M. Sorice;L. Lenti;R. Misasi;C. Contini;L. Cignarella;T. Griggi;V. Vullo;C. Masala
本研究旨在评估糖脂抗原（特别是神经节苷脂）是否与从人肺中获得的卡氏肺孢子虫相关。提取神经节苷脂，用高效薄层色谱法纯化，用间苯二酚染色。结果表明，卡氏肺吸虫存在与GM1和GD1a共迁移的两条间苯二酚阳性带，提示卡氏肺吸虫存在神经节苷脂分子。在肺对照组织中未发现间苯二酚阳性条带。此外，从卡氏肺孢子虫抗原免疫的兔获得的抗血清与神经节苷脂GM1和GD1a反应，如斑点免疫结合试验所示。这种反应性被抑制，首先孵育的抗血清与神经节苷脂胶束。此外，抗鞘糖脂抗体（aGM 1）与卡氏肺孢子虫抗原的200和55 kDa条带反应。这些结果表明，卡氏肺吸虫表达的神经节苷脂抗原可以引发特异性免疫应答。
This study was undertaken to assess whether glycolipid antigens (particularly gangliosides) are associated with Pneumocystis carinii obtained from human lungs. Gangliosides were extracted, purified in high performance thin-layer chromatography and stained with resorcinol. Two resorcinol-positive bands, co-migrating with GM1 and GD1a were demonstrated, suggesting the existence of ganglioside molecules on P. carinii. No resorcinol-positive bands were revealed in the pulmonary control tissue. In addition, an antiserum obtained from rabbits immunized with P. carinii antigen reacted with gangliosides GM1, and GD1a, as revealed by a dot immunobinding assay. This reactivity was inhibited by first incubating the antiserum with ganglioside micelles. Furthermore, anti-glycosphingolipid antibodies (aGM1) reacted with the bands of 200 and 55 kDa of P. carinii antigen. These results suggest that ganglioside antigens expressed on P. carinii can trigger specific immune responses.
卡氏肺囊虫的糖蛋白：通过电泳和显微镜进行表征。
DOI：
10.1093/infdis/158.6.1353
发表时间：
1988
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Pesanti,EL;Shanley,JD
通讯作者：
Shanley,JD