Design characteristics for the tissue engineering of cartilaginous tissues
Design characteristics for the tissue engineering of cartilaginous tissues
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DOI：
10.1023/b:abme.0000007786.37957.65
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发表时间：
2004-01-01
影响因子：
3.8
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Almarza, AJ;Athanasiou, KA
颞下颌关节（TMJ）椎间盘和膝关节半月板等组织经常被误认为等同于透明软骨，这在很大程度上是由于对软骨组织的区别特性缺乏全面的了解。由于这种混淆，纤维软骨组织工程的尝试可能不会基于合适的实验设计。纤维软骨组织与透明软骨明显不同；然而，与它们的细胞和生化组成以及生物力学特征相关的知识匮乏令人震惊。透明关节软骨完全由软骨细胞组成，主要产生11型胶原蛋白，而TMJ椎间盘和膝关节半月板有成纤维细胞和类似软骨细胞的混合细胞群，主要分泌I型胶原蛋白。此外，与透明软骨相比，纤维软骨组织具有低糖胺聚糖含量、低压缩模量和高拉伸模量。因此，纤维软骨组织工程的尝试是组织特异性的，利用这些组织的独特特性的知识是至关重要的。同时，纤维软骨组织的体外工程化必须认真考虑透明软骨再生的科学和工程方面的进展和见解。
Tissues like the temporomandibular joint (TMJ) disc and the knee meniscus are often mistakenly viewed as a tantamount to hyaline cartilage, largely due to the absence of a comprehensive understanding of the distinguishing properties of cartilaginous tissues. Because of this confusion, fibrocartilaginous tissue engineering attempts may not be based on suitable experimental designs. Fibrocartilaginous tissues are markedly different than hyaline cartilage; however, the dearth of knowledge related to their cellular and biochemical composition, as well as their biomechanical characteristics, is stunning. Hyaline articular cartilage is exclusively composed of chondrocytes that produce primarily type 11 collagen, whereas the TMJ disc and the knee meniscus have a mixed cell population of fibroblasts and cells similar to chondrocytes, which predominantly secrete type I collagen. Additionally, fibrocartilaginous tissues have a low glycosaminoglycan content, a low compressive modulus, and a high tensile modulus when compared to hyaline cartilage. Therefore, it is crucial for fibrocartilaginous tissue engineering attempts to be tissue-specific, utilizing the knowledge of the distinct and unique properties of these tissues. At the same time, advances and insights related to the science and engineering aspect of hyaline cartilage regeneration must be carefully considered for the in vitro engineering of fibrocartilaginous tissues.