Hepatic maturation in differentiating embryonic stem cells in vitro
Hepatic maturation in differentiating embryonic stem cells in vitro
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DOI：
10.1016/s0014-5793(01)02423-1
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发表时间：
2001-05-18
期刊：
影响因子：
3.5
通讯作者：
Terada, N
中科院分区：
文献类型：
--
作者：
Hamazaki, T;Iiboshi, Y;Terada, N
我们研究了小鼠胚胎干细胞（ES）在体外向肝细胞分化的潜力。分化的ES细胞表达内胚层特异性基因，如甲胎蛋白、甲状腺素运载蛋白、α 1-抗胰蛋白酶和白蛋白，当在没有额外的生长因子和肝发育的晚期分化标志物如酪氨酸转氨酶（达特）和葡萄糖-6-磷酸酶（G6 P）的情况下培养时。当在存在对晚期胚胎肝发育至关重要的生长因子的情况下培养时。此外，达特和G6 P表达的诱导与功能性SEK 1基因的表达无关，其被认为在肝形态发生的早期阶段为肝细胞提供存活信号。数据表明体外ES分化系统具有产生成熟肝细胞的潜力。该系统还被发现可用于分析生长因子和细胞内信号分子在肝脏发育中的作用。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
We investigated the potential of mouse embryonic stem (ES) cells to differentiate into hepatocytes in vitro. Differentiating ES cells expressed endodermal-specific genes, such as alpha -fetoprotein, transthyretin, a l-anti-trypsin and albumin, when cultured without additional growth factors and late differential markers of hepatic development, such as tyrosine aminotransferase (TAT) and glucose-6-phosphatase (G6P). when cultured in the presence of growth factors critical for late embryonic liver development. Further, induction of TAT and G6P expression was induced regardless of expression of the functional SEK1 gene, which is thought to provide a survival signal for hepatocytes during an early stage of liver morphogenesis, The data indicate that the in vitro ES differentiation system has a potential to generate mature hepatocytes. The system has also been found useful in analyzing the role of growth factors and intracellular signaling molecules in hepatic development. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.