B- and T-lymphocyte responses to an immunodominant epitope of human immunodeficiency virus.
B- and T-lymphocyte responses to an immunodominant epitope of human immunodeficiency virus.
复制标题
B 和 T 淋巴细胞对人类免疫缺陷病毒免疫显性表位的反应。
DOI：
10.1128/jvi.62.8.2531-2536.1988
复制
发表时间：
1988
影响因子：
5.4
通讯作者：
Oldstone,MB
中科院分区：
文献类型：
--
作者：
Schrier,RD;GnannJr,JW;Langlois,AJ;Shriver,K;Nelson,JA;Oldstone,MB
利用合成的多肽，我们研究了人类免疫缺陷病毒1型(HIV-1)跨膜糖蛋白(env氨基酸，598-609)氨基末端附近的免疫优势表位对B淋巴细胞(抗体)和T淋巴细胞(增殖)的反应。针对该表位的免疫球蛋白M(IgM)和免疫球蛋白G抗体均在初次感染HIV-1后早期出现。在动物模型中，从该序列衍生的合成肽的免疫球蛋白G反应是T辅助细胞依赖的，而免疫球蛋白M反应是T细胞非依赖的。此外，用该多肽免疫产生的抗体具有HIV-1中和活性。在203名感染HIV-1的患者中，超过99%(201人)在体内产生了对这种多肽的抗体；然而，只有24%(7/29)的患者在体外对这种多肽产生了增殖的T细胞。这些观察结果表明，不同的HIV-1gp41表位可以激发B细胞和T细胞的免疫反应。
Using synthetic peptides, we characterized the B-lymphocyte (antibody) and T-lymphocyte (proliferation) responses to an immunodominant epitope of human immunodeficiency virus type 1 (HIV-1) located near the amino-terminal end of the transmembrane glycoprotein (env amino acids 598 to 609). Both immunoglobulin M (IgM) and IgG antibodies against this epitope appeared early after primary infection with HIV-1. In an animal model, the IgG response to a synthetic peptide derived from this sequence was T-helper-cell dependent, whereas the IgM response was T-cell independent. In addition, antibody generated by immunization with this peptide had HIV-1-neutralizing activity. Greater than 99% (201 of 203) of patients infected with HIV-1 generated antibody to this peptide in vivo; however, only 24% (7 of 29) had T cells that proliferated in response to this peptide in vitro. These observations suggest that different HIV-1 gp41 epitopes elicit B-cell and T-cell immune responses.