Suppression of an elicitor-induced oxidative burst reaction in Medicago sativa cell cultures by Sinorhizobium meliloti lipopolysaccharides
Suppression of an elicitor-induced oxidative burst reaction in Medicago sativa cell cultures by Sinorhizobium meliloti lipopolysaccharides
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DOI：
10.1046/j.0028-646x.2001.00214.x
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发表时间：
2001-09-01
期刊：
影响因子：
9.4
通讯作者：
Niehaus, K
中科院分区：
文献类型：
--
作者：
Albus, U;Baier, R;Niehaus, K
本文研究了苜蓿中华根瘤菌（Sinorhizobium meliloti）的脂多糖（LPS）在寄主植物紫花苜蓿（Medicago sativa）和非寄主植物烟草（Nicotiana tabacum）细胞培养中的生物活性。苜蓿草被纯化和化学表征。拟南芥和烟草悬浮细胞培养响应酵母诱导剂与碱化的培养基和诱导的氧化爆发。在苜蓿细胞培养中，同时加入纯化的S.苜蓿抑制诱导子诱导的碱化反应和氧化猝发反应。非寄主烟草的细胞培养物对S.苜蓿属植物唇。在这些细胞培养物中，S.苜蓿LIPS本身引起培养基的碱化和氧化爆发反应。苜蓿根瘤菌LPS可能作为一种特异性信号分子，促进共生互作，抑制寄主植物苜蓿的致病反应。(C)新植物学家（2001）。
The biological activity of lipopolysaccharides (LPS) from the symbiotic soil bacterium Sinorhizobium meliloti was analysed in cell cultures of the host plant Medicago sativa (alfalfa) and the nonhost plant Nicotiana tabacum (tobacco).LPS of S. meliloti were purified and chemically characterized. Alfalfa and tobacco suspension cell cultures responded to yeast elicitors with an alkalinization of the culture medium and the induction of an oxidative burst. This assay was used to study the biological activity of isolated LPS.In alfalfa cell cultures the simultaneous addition of purified LPS of S. meliloti suppressed the elicitor induced alkalinization and oxidative burst reaction. Cell cultures of the nonhost tobacco reacted differently to the application of S. meliloti LIPS. In these cell cultures, the S. meliloti LIPS itself caused an alkalinization of the culture medium and an oxidative burst reaction.S. meliloti LPS released from the bacterial surface might function as a specific signal molecule, promoting the symbiotic interaction and suppressing a pathogenic response in the host plant, alfalfa. (C) New Phytologist (2001).