IS-10 MESSENGER-RNA STABILITY AND STEADY-STATE LEVELS IN ESCHERICHIA-COLI - INDIRECT EFFECTS OF TRANSLATION AND ROLE OF RNE FUNCTION
IS-10 MESSENGER-RNA STABILITY AND STEADY-STATE LEVELS IN ESCHERICHIA-COLI - INDIRECT EFFECTS OF TRANSLATION AND ROLE OF RNE FUNCTION
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DOI：
10.1111/j.1365-2958.1993.tb01686.x
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发表时间：
1993-07-01
影响因子：
3.6
通讯作者：
KLECKNER, N
中科院分区：
文献类型：
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作者：
JAIN, C;KLECKNER, N
已知IS 10转座酶基因的翻译非常罕见。我们已经确定了突变的遗传特性表明，它们直接作用于增加或减少翻译起始的内在水平。此外，我们详细分析了这些突变对IS 10 mRNA的影响，使用一个特定的IS 10衍生物。在这种情况下，翻译的增加或减少伴随着转座酶mRNA的稳态水平和半衰期的增加或减少;对稳态水平的影响比对信息半衰期的影响更显着。在翻译起始的野生型水平下，特定IS 10衍生物的全长IS 10信息的物理衰变中的限速步骤显示为核酸依赖性内切核酸酶裂解; 3'核酸外切酶似乎起次要作用，降解初级裂解产物。翻译突变和me功能之间的相互作用的分析，以及上述观察结果，表明翻译稳定的信息，在一般的方式对rne依赖性内切核酸裂解，并显着的保护可能是由一个或几个核糖体。然而，戏剧性的影响，稳定状态的信息水平上的翻译仍然观察到在我突变体，并涉及3'端的转录，我们建议，这些额外的影响反映了转录本释放的抑制介导的刺激。
Translation of the IS10 transposase gene is known to be very infrequent. We have identified mutations whose genetic properties suggest that they act directly to increase or decrease the intrinsic level of translation initiation. Also, we have analysed in detail the effects of these mutations on IS10 mRNA using one particular IS10 derivative. In this case, increases or decreases in translation are accompanied by increases or decreases in both the steady state level and the half-life of transposase mRNA; effects on steady state levels are much more dramatic than effects on message half-life. At wild-type levels of translation initiation, the rate-limiting step in physical decay of full length IS10 message for a particular IS10 derivative is shown to be rne-dependent endonucleolytic cleavage; 3' exonucleases appear to play a secondary role, degrading primary cleavage products. Analysis of interplay between translation mutations and me function, together with the above observations, suggests that translation stabilizes messages in a general way against rne-dependent endonucleolytic cleavage , and that significant protection may be conferred by one or a few ribosomes. However, dramatic effects of translation on steady state message levels are still observed in an me mutant and involve the 3' end of the transcript; we propose that these additional effects reflect translation-mediated stimulation of transcript release.