High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections
High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections
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DOI：
10.1016/j.ijms.2006.09.028
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发表时间：
2007-02-01
影响因子：
1.8
通讯作者：
Piersma, Sander R.
中科院分区：
文献类型：
--
作者：
Altelaar, A. F. Maarten;Taban, Ioana M.;Piersma, Sander R.
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）已被用于直接测定大鼠、小鼠和人垂体组织切片上的肽分布。由于这些器官很小(10(2)-10(3)μ m)， IMS的空间分辨率是垂体组织切片分子成像的关键问题。在这里，我们展示了高分辨率IMS允许在垂体组织切片的单个器官的不同细胞簇内定位神经肽分布。样品制备方案不会导致分析物重新分布，因此适用于细胞长度尺度的IMS实验。本研究中使用的污名成像质谱仪产生的选择离子计数图像像素尺寸为500 nm，分辨率为4 μ m，与微探针成像实验获得的图像相比，具有更好的空间细节。此外，我们表明，通过成像质谱法可以根据具有相同功能的神经肽的氨基酸序列的差异来区分不同的哺乳动物组织切片。这个例子展示了IMS在相关生物长度尺度上进行无标记分子成像的能力。(C) 2006 Elsevier B.V.版权所有
Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has been used to determine peptide distributions directly from rat, mouse and human pituitary tissue sections. Since these organs are small (10(2)-10(3) mu m) the spatial resolution of IMS is a key issue in molecular imaging of pituitary tissue sections. Here we show that high-resolution IMS allows localization of neuropeptide distributions within different cell clusters of a single organ of a pituitary tissue section. The sample preparation protocol does not result in analyte redistribution and is therefore applicable to IMS experiments at cellular length scales. The stigmatic imaging mass spectrometer used in this study produces selected-ion-count images with pixel sizes of 500 nm and a resolving power of 4 mu m, yielding superior spatial detail compared to images obtained in microprobe imaging experiments. Furthermore, we show that with imaging mass spectrometry a distinction can be made between different mammalian tissue sections based on differences in the amino acid sequence of neuropeptides with the same function. This example demonstrates the power of IMS for label-free molecular imaging at relevant biological length scales. (C) 2006 Elsevier B.V. All rights reserved.