Development of diagnostic reagents to differentiate between Mycobacterium bovis BCG vaccination and M-bovis infection in cattle
Development of diagnostic reagents to differentiate between Mycobacterium bovis BCG vaccination and M-bovis infection in cattle
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DOI：
10.1128/cdli.6.5.675-682.1999
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发表时间：
1999-09-01
期刊：
影响因子：
--
通讯作者：
Hewinson, RG
中科院分区：
文献类型：
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作者：
Vordermeier, HM;Cockle, PC;Hewinson, RG
在英国，政府最近的一项独立科学审查得出结论，开发牛分枝杆菌疫苗是英国牛群结核病控制的最佳长期前景。疫苗接种的必要条件是开发一种补充诊断试验，以区分接种疫苗的动物和感染M.因此，以测试和屠宰为基础的控制策略可以与疫苗接种一起继续。为了评估开发活疫苗鉴别诊断试验的可行性，我们选择了M。Bovis BCG Pasteur作为模型系统。在M.在BCG巴斯德（ESAT-6、MPB 64、MPB 70和MPBS 3）中，产生了牛的，但没有产生或仅以低水平产生。这些试剂通过使用来自M.牛感染、BCG接种和鸟分枝杆菌致敏小牛。所有四种抗原仅在M中诱导体外增殖和γ干扰素应答。牛感染的动物。由ESAT-6、MPB 64和MPB 83组成的混合物鉴定了感染的动物，但未鉴定接种BCG的动物。此外，将ESAT-6、MPB 64和MPB 83的混杂T细胞表位配制成肽混合物。在用这种肽混合物进行的T细胞试验中，感染动物的鉴定频率与用蛋白混合物进行的试验中获得的频率相似，而BCG接种或M.禽流感致敏的动物没有反应。总之，我们的研究结果表明，肽和蛋白质的鸡尾酒可以被设计来区分M。牛感染和卡介苗接种。
In Great Britain a recent independent scientific review far the government has concluded that the development of a cattle vaccine against Mycobacterium bovis holds the best long-term prospect for tuberculosis control in British herds. A sine qua non for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated animals and those infected with M. bovis so that test-and-slaughter-based control strategies can continue alongside vaccination. In order to assess the feasibility of developing a differential diagnostic test for a live vaccine, we chose M. bovis BCG Pasteur as a model system. Recombinant forms of antigens which are expressed in M. bovis but not, or only at low levels, in BCG Pasteur (ESAT-6, MPB64, MPB70, and MPBS3) were produced. These reagents were tested either alone or in combination by using peripheral blood mononuclear cells from M. bovis-infefted, BCG-vaccinated, and Mycobacterium avium-sensitized calves. All four antigens induced in vitro proliferation and gamma interferon responses only in M. bovis-infected animals. A cocktail composed of ESAT-6, MPB64, and MPB83 identified infected animals but not those vaccinated with BCG. In addition, promiscuous T-cell epitopes of ESAT-6, MPB64, and MPB83 were formulated into a peptide cocktail. In T-cell assays with this peptide cocktail, infected animals were identified with frequencies similar to those obtained in assays with the protein cocktail, while BCG-vaccinated or M. avium-sensitized animals did not respond. In summary, our results suggest that peptide and protein cocktails can be designed to discriminate between M. bovis infection and BCG vaccination.