Chronic riluzole treatment increases glucose metabolism in rat prefrontal cortex and hippocampus
Chronic riluzole treatment increases glucose metabolism in rat prefrontal cortex and hippocampus
复制标题
DOI：
10.1038/jcbfm.2008.78
复制
发表时间：
2008-12-01
影响因子：
6.3
通讯作者：
Sanacora, Gerard
中科院分区：
文献类型：
--
作者：
Chowdhury, Golam M. I.;Banasr, Mounira;Sanacora, Gerard
阿曲唑被认为通过减少谷氨酸释放和促进星形胶质细胞摄取来调节谷氨酸能功能。我们在用利鲁唑（21天，每天4 mg/kg，i. p.）或盐水。使用H-1-[C-13] NMR光谱法测定提取物中代谢物的总浓度和C-13浓度。在前额叶皮层（P < 0.05）和海马（P < 0.05）中，利鲁唑增加了谷氨酸-C4（至112%和130%）、GABA-C2（至142%和171%）和谷氨酰胺-C4（至118%和233%）的C-13标记，而不影响总代谢物水平（P > 0.2）。我们的研究结果表明，与预期相反，慢性利鲁唑增强葡萄糖氧化代谢和谷氨酸/谷氨酰胺循环。
Riluzole is believed to modulate glutamatergic function by reducing glutamate release and facilitating astroglial uptake. We measured C-13 labeling in metabolites in prefrontal cortex and hippocampus during a 10 mins infusion of [1-C-13] glucose in urethane anesthetized rats treated with riluzole (21 days, 4 mg/kg per day, i.p.) or saline. Total and C-13 concentrations of metabolites were determined in extracts using H-1-[C-13] NMR spectroscopy. In prefrontal cortex (P < 0.05) and hippocampus (P < 0.05) riluzole increased C-13 labeling over saline in glutamate-C4 (to 112% and 130%), GABA-C2 (to 142% and 171%), and glutamine-C4 (to 118% and 233%) without affecting total metabolite levels (P > 0.2). Our findings indicate that contrary to expectation chronic riluzole enhanced glucose oxidative metabolism and glutamate/glutamine cycling.