Cytogenetics of Amaryllidaceae species: heterochromatin evolution in different ploidy levels
Cytogenetics of Amaryllidaceae species: heterochromatin evolution in different ploidy levels
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DOI：
10.1007/s00606-011-0418-2
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发表时间：
2011-02
影响因子：
1.9
通讯作者：
W. Felix;L. Felix;N. Melo;J. H. Dutilh;Reginaldo de Carvalho
中科院分区：
文献类型：
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作者：
W. Felix;L. Felix;N. Melo;J. H. Dutilh;Reginaldo de Carvalho
用色霉素A3（CMA）/4，6-二脒基-2-苯基吲哚（DAPI）荧光染料对石蒜科（ZephyranthesandHabranthus）的物种进行了显带分析。本文研究了七种泽兰的带型。和一株哈伯兰瑟斯草在Z中观察到亚末端和间质DAPI+带。robusta 2n = 12和Z. brachyandra2n = 24。其他物种显示没有AT丰富的异染色质。在2n = 12的种中，在Z的一对染色体上观察到CMA+带。RobustaandZephyranthessp.，而在Z。在第五对染色体上观察到了额外的小末端带。rosea和Z. grandiflora的2n = 24，有4条CMA+带，而Z.短蕊花，2n = 24 + 1 B，B染色体短臂上有8条间质点带和一条较大的末端带。2n = 38的假丝酵母在5对中部着丝粒对的长臂和其中一对亚中部着丝粒对的短臂上出现CMA+异染色质块，另外在一对较大的亚中部着丝粒对的一个同源物的长臂上观察到一条终带。itaobinus显示了一个杂合对，仅在一个同源物中显示出强烈的CMA+带，可能是核仁组织区。分类学意义和核型进化的这组进行了讨论，并与以前的数据从文献中。
Species belonging to the Amaryllidaceae (ZephyranthesandHabranthus) were analyzed by banding with chromomycin A3 (CMA)/4,6-diamidino-2-phenylindole (DAPI) fluorochromes. The patterns of bands were studied in seven species ofZephyranthesHerb. and one ofHabranthusHerb. Subterminal and interstitial DAPI+ bands were observed inZ. robusta2n = 12 andZ. brachyandra2n = 24. Other species showed no AT-rich heterochromatin. In species with 2n = 12, CMA+ bands were observed on one chromosome pair ofZ. robustaandZephyranthessp., while inZ. sylvaticaan additional small terminal band in the fifth chromosome pair was observed.Z. roseaandZ. grandiflorapresented with 2n = 24 and had four CMA+ bands, while inZ. brachyandra, with 2n = 24 + 1B, there were eight interstitial dot bands and a larger terminal band in the short arm of the B chromosome.Z. candidawith 2n = 38 presented CMA+ heterochromatin blocks on the long arms of five metacentric pairs and in the short arm of one of the submetacentric pairs; in addition a terminal band was observed on the long arm of one of the homologues of a larger submetacentric pair.H. itaobinusshowed a heterozygous pair revealing a strong CMA+ band in only one of the homologues, likely a nucleolus organizing region. Taxonomic implications and karyotype evolution of this group are discussed and correlated with previous data from the literature.