PHYSIOLOGICAL AND MORPHOLOGICAL IDENTIFICATION OF HORIZONTAL, BIPOLAR AND AMACRINE CELLS IN GOLDFISH RETINA
PHYSIOLOGICAL AND MORPHOLOGICAL IDENTIFICATION OF HORIZONTAL, BIPOLAR AND AMACRINE CELLS IN GOLDFISH RETINA
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DOI：
10.1113/jphysiol.1970.sp009084
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发表时间：
1970-01-01
影响因子：
5.5
通讯作者：
KANEKO, A
中科院分区：
文献类型：
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作者：
KANEKO, A
1.从离体金鱼视网膜中的各种类型的细胞进行细胞内记录，并从记录移液管注射Procion Yellow以在组织学上鉴定记录的结构。染料扩散并染色细胞体和突起，直至细支。S电位被确定为来自外部水平细胞和内部水平细胞。在两个区域均发现L型和C型S电位，并且在给予这两种类型反应的细胞中未观察到组织学差异。从外部水平细胞记录的S-电位比从内部水平细胞记录的S-电位显示更少的空间总和。双极细胞反应由与拮抗性中心-环绕型感受野（中心上、非环绕或相反）相关的持续电位组成。在双极细胞中未观察到锋电位活性.无长突细胞在光照开始和结束时都表现出短暂的去极化。它们有时表现出尖峰活动。开-和关-去极化的幅度显示出对刺激几何形状的轻微依赖性，但未观察到明显的中心-环绕组织。
1. Intracellular recordings were made from various types of cells in the isolated goldfish retina, and Procion Yellow was injected from the recording pipette in order to identify histologically the structure recorded. The dye diffused and stained the cell body and processes down to the fine branches.2. S‐potentials were identified as coming from the external horizontal cells and from the internal horizontal cells. Both L‐ and C‐type S‐potentials were found in both regions, and no histological differences were seen in cells giving these two types of responses. S‐potentials recorded from the external horizontal cells showed less spatial summation than those recorded from the internal horizontal cells.3. Bipolar cell responses consisted of sustained potentials associated with an antagonistic centre‐surround type receptive field (on‐centre, off‐surround or vice versa). Spike activity was not observed in bipolar cells.4. Amacrine cells responded with transient depolarization both at the beginning and at the end of illumination. They sometimes showed spike activity. The amplitude of on‐ and off‐depolarization showed slight dependence on stimulus geometry but a distinct centre‐surround organization was not observed.