Development of a sensitive LC-MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection
Development of a sensitive LC-MS/MS method for simultaneous quantification of eleven constituents in rat serum and its application to a pharmacokinetic study of a Chinese medicine Shengmai injection
复制标题
建立同时定量大鼠血清中11种成分的灵敏LC-MS/MS方法及其在中药生脉注射液药代动力学研究中的应用
DOI：
10.1002/bmc.3273
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发表时间：
2015-02-01
影响因子：
1.8
通讯作者：
Li, Zheng
中科院分区：
文献类型：
--
作者：
Zhan, Shuyu;Shao, Qing;Li, Zheng
以地高辛为内标，建立了同时测定大鼠血清中五味子醇甲、五味子醇甲、五味子醇B和五味子醇B、麦冬皂苷D、人参皂苷Rg 1、Re、Rf、Rg 2、Rb 1、Rd、Rc等11种成分的LC-MS/MS方法。血清样品经预处理和两步液液萃取。采用C-18色谱柱，以0.02%乙酸水溶液和0.02%乙酸-乙腈为移动的流动相进行梯度洗脱，流速为0.5 mL/min，电喷雾离子源多反应监测，质谱检测。在验证的浓度范围内，每种分析物均观察到良好的线性（r（2）0.9929），大鼠血清中分析物的定量下限范围为0.044-1.190 ng/mL。日内和日间精密度均为75.8%。版权所有（c）2014约翰威利父子有限公司
A sensitive LC-MS/MS method was developed and validated for simultaneous quantification of 11 constituents, ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rd, Rc, ophiopogonin D, schisandrin, schisandrol B and schizandrin B, in rat serum using digoxin as the internal standard (IS). The serum samples were pretreated and extracted with a two-step liquid-liquid extraction. Chromatographic separation was achieved on a C-18 analytical column with a proper gradient elution using 0.02% acetic acid aqueous solution and 0.02% acetic acid-acetonitrile as mobile phase at a flow rate of 0.5 mL/min. MS detection was performed using multiple reaction monitoring via an electrospray ionization source. Good linearity was observed in the validated concentration range for every analyte (r(2) 0.9929), and the lower limits of quantitation of the analytes were in the range of 0.044-1.190 ng/mL in rat serum. Intra- and inter-day precisions were 75.8%.The validated method was successfully applied to a pharmacokinetic study of all analytes in rats after single intravenous administration of Shengmai injection. Copyright (c) 2014 John Wiley & Sons, Ltd.