Association between the HLA genotype and the severity of COVID-19 infection among South Asians
Association between the HLA genotype and the severity of COVID-19 infection among South Asians
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DOI：
10.1002/jmv.27003
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发表时间：
2021-04-23
影响因子：
12.7
通讯作者：
Al-nazawi，Ashwaq
中科院分区：
文献类型：
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作者：
Naemi，Fatmah M. A.;Al-adwani，Shurooq;Al-nazawi，Ashwaq
COVID - 19感染的发病率和严重程度存在区域差异。人白细胞抗原（HLA）多态性是可能影响该病预后的遗传因素之一。本研究探讨了南亚患者HLA基因型与COVID - 19严重程度之间的关系。收集了95名患有COVID - 19的亚洲人（孟加拉国、印度和巴基斯坦人）的血液样本。根据感染的严重程度将患者分为轻度（64例）、重度（31例）和致命（20例）。从所有样品中提取DNA，并采用PCR - rSSO（聚合酶链反应-反序列特异性寡核苷酸）分子方法对I类（A、B和C）和II类（DRB1、DQA1和DQB1）进行HLA基因分型。死亡组患者HLA - B*51阳性率明显高于轻度感染组（15% vs. 4.7%,p= 0.027）。此外，HLA - B*35在轻度感染组的频率明显高于死亡组（21.1% vs. 7.5%,p= 0.050 [a borderlinep‐value]）。HLAⅱ类DRB1*13在死亡组显著高于轻度感染组（17.5%比11.3%,p= 0.049）。然而，在对p值进行统计校正后，所有等位基因的p值都变得不显著（pc= 0.216,pc= 0.4和pc= 0.49）。与所有已发表的数据相比，该研究强调HLA系统与COVID - 19结局之间的关联可能与种族相关。必须在更广泛的范围内检查人群之间的遗传变异，以评估感染预后和疫苗有效性。
Regional variations are found in the incidence and severity of the COVID‐19 infection. Human leukocyte antigen (HLA) polymorphism is one of the genetic factors that might have an impact on the outcome of the disease. This study explored the association between the HLA genotype and the severity of COVID‐19 among patients from South Asia. Blood samples from 95 Asians (Bangladeshis, Indians, and Pakistanis) with COVID‐19 were collected. The patients were divided according to the severity of their infection: mild (N= 64), severe (N= 31), and fatal (N= 20). DNA was extracted from all samples, and HLA genotyping was performed for both class I (A, B, and C) and class II (DRB1, DQA1, and DQB1) using the PCR‐rSSO (polymerase chain reaction–reverse sequence‐specific oligonucleotide) molecular method. The frequency of HLA‐B*51 was significantly higher among patients in the fatal group than among those in the mild infection group (15% vs. 4.7%,p= 0.027). Additionally, the frequency of HLA‐B*35 was significantly higher in the mild infection group than in the fatal group (21.1% vs. 7.5%,p= 0.050 [a borderlinep‐value]). In terms of HLA class II, DRB1*13 was significantly observed in the fatal group than in the mild infection group (17.5% vs. 11.3%,p= 0.049). However, thep‐value for all alleles became insignificant after a statistical correction for thep‐values (pc= 0.216,pc= 0.4, andpc= 0.49, respectively). Compared with all published data, this study highlights that the association between the HLA system and the COVID‐19 outcome might be ethnic‐dependent. Genetic variation between populations must be examined on a wider scale to assess infection prognosis and vaccine effectiveness.