Selective induction of human beta-defensin mRNAs by Actinobacillus actinomycetemcomitans in primary and immortalized oral epithelial cells.
Selective induction of human beta-defensin mRNAs by Actinobacillus actinomycetemcomitans in primary and immortalized oral epithelial cells.
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DOI：
10.1046/j.0902-0055.2002.00097.x
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发表时间：
2003-12
影响因子：
--
通讯作者：
E. Feucht;C. L. Desanti;Aaron Weinberg
中科院分区：
文献类型：
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作者：
E. Feucht;C. L. Desanti;Aaron Weinberg
人β-防御素-2和-3（hBD-2，-3）是参与宿主防御的小的可诱导的抗微生物肽。伴放线放线杆菌是一种革兰氏阴性兼性厌氧菌，经常与人类口腔疾病有关。A.检测伴放线菌菌株JP 2调节正常人口腔上皮细胞（NHOEC）和OKF 6/Tert细胞（来源于人口腔上皮细胞的永生化细胞系）中hBD-2和hBD-3基因表达的能力。活细菌以最小细菌/细胞比500：1刺激两种细胞类型，导致hBD-3基因表达增加。即使在细菌/细胞比超过500：1的情况下，在具有活细菌的两种细胞类型中的hBD-2也未表现出这一点。hBD-3基因表达的增加依赖于活菌，而不是它们的脂多糖（LPS），因为热杀死了A。伴放线菌不诱导hBD-3转录本的表达。在OKF 6/Tert细胞和NHOEC中获得的结果之间的总体相似性表明，OKF 6/Tert细胞系可能是研究口腔上皮中β-防御素表达的有用工具。
Human beta-defensin-2, and -3 (hBD-2, -3) are small inducible antimicrobial peptides involved in host defense. Actinobacillus actinomycetemcomitans, a gram-negative facultative anaerobe, is frequently associated with oral disease in humans. A. actinomycetemcomitans, strain JP2, was examined for its ability to modulate hBD-2 and -3 gene expression in normal human oral epithelial cells (NHOECs) and in OKF6/Tert cells, an immortalized cell line derived from human oral epithelial cells. Stimulation of both cell types by live bacteria, at a minimal bacteria/cell ratio of 500 : 1, resulted in increased hBD-3 gene expression. This was not evinced for hBD-2 in either cell type with live bacteria, even at bacteria/cell ratios exceeding 500 : 1. The increased hBD-3 gene expression was dependent upon viable bacteria, and not their lipopolysaccharides (LPS), since heat-killed A. actinomycetemcomitans did not induce hBD-3 transcript expression. The overall similarity between results obtained in OKF6/Tert cells and NHOECs suggest that the OKF6/Tert cell line may be a useful tool in the study of beta-defensin expression in oral epithelium.