Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility
Molecular characterization of the CER1 gene of arabidopsis involved in epicuticular wax biosynthesis and pollen fertility
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DOI：
10.1105/tpc.7.12.2115
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发表时间：
1995-12-01
期刊：
影响因子：
11.6
通讯作者：
Pereira, A
中科院分区：
文献类型：
--
作者：
Aarts, MGM;Keijzer, CJ;Pereira, A
植物的地上部分被覆盖有上表皮蜡层，这是重要的作为抵御外部影响的第一道防线。在拟南芥中，ECERIFERUM（CER）基因影响蜡生物合成途径的不同步骤。在这篇文章中，我们描述了CER 1基因的分离，该基因编码一种新的蛋白质，该蛋白质参与长链醛转化为烷烃，这是蜡生物合成的关键步骤。CER 1是在用异源玉米转座因子系统增强子-抑制子（也称为抑制子-突变子）进行基因标记后克隆的。cer 1突变体显示有光泽的绿色茎和果实，并且是条件雄性不育的。CER 1蛋白质与一组处理高度疏水分子的膜酶的相似性表明CER 1蛋白质具有脱羧酶的功能。
The aerial parts of plants are coated with an epicuticular wax layer, which is important as a first line of defense against external influences, In Arabidopsis, the ECERIFERUM (CER) genes effect different steps of the wax biosynthesis pathway. In this article, we describe the isolation of the CER1 gene, which encodes a novel protein involved in the conversion of long chain aldehydes to alkanes, a key step in wax biosynthesis. CER1 was cloned after gene tagging with the heterologous maize transposable element system Enhancer-inhibitor, also known as Suppressor-mutator. cer1 mutants display glossy green stems and fruits and are conditionally male sterile. The similarity of the CER1 protein with a group of integral membrane enzymes, which process highly hydrophobic molecules, points to a function of the CER1 protein as a decarbonylase.