Molecular mapping of genetic and chromomeric units in Drosophila melanogaster.
Molecular mapping of genetic and chromomeric units in Drosophila melanogaster.
复制标题
果蝇遗传和染色体单位的分子图谱。
DOI：
10.1016/s0022-2836(83)80321-0
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发表时间：
1983
影响因子：
5.6
通讯作者：
P. Chambon
中科院分区：
文献类型：
--
作者：
P. Spierer;A. Spierer;Welcome Bender;D. Hogness;P. Chambon
我们使用了一组重叠的克隆片段来定义果蝇黑腹染色体DNA的315 kb(×103个碱基对)区域，以定位与多线带-带间(染色单体)相关的序列以及该区域内包含的致死互补基团。用所选片段与多线染色体进行原位杂交，确定了第三染色体87D5-6至87E5，6区13±1个染色单体的分子图谱位置。315kb区域内最大的染色单体的长度约为160kb，而最小的染色单体的长度小于7kb，可短至3kb。通过定位315kb区域内的缺失断裂点，我们定位了它的12个致死互补基团，其中包括编码乙酰胆碱酯酶(Ace)和黄嘌呤脱氢酶(ROSY)的基因。两个分子图谱的比较表明，遗传单位和染色单体之间存在一对一的地形关联。
We have used a set of overlapping cloned segments defining a 315 kb (×103basepairs) region ofDrosophila melanogasterchromosomal DNA to map the sequences associated with the polytene band-interbands (chromomeric units) and with the lethal complementation groups contained within this region. The molecular map positions of the 13±1 chromomeric units from the 87D5-6 to 87E5, 6 region of the third chromosome were determined byin situhybridization of selected segments to the polytene chromosomes. The length of the largest chromomeric unit within the 315 kb region is approximately 160 kb, while that for the smallest is less than 7 kb and may be as short as 3 kb. By mapping the breakpoints of deletions within the 315 kb region, we have located its 12 lethal complementation groups, which include the genes coding for acetylcholinesterase (Ace) and xanthine dehydrogenase (rosy). Comparison of the two molecular maps indicates a one-to-one topographical correlation between the genetic and chromomeric units.