The utility of Plasmodium berghei as a rodent model for anti-merozoite malaria vaccine assessment.
The utility of Plasmodium berghei as a rodent model for anti-merozoite malaria vaccine assessment.
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DOI：
10.1038/srep01706
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发表时间：
2013
影响因子：
4.6
通讯作者：
Draper, Simon J.
中科院分区：
文献类型：
--
作者：
Goodman, Anna L.;Forbes, Emily K.;Williams, Andrew R.;Douglas, Alexander D.;de Cassan, Simone C.;Bauza, Karolis;Biswas, Sumi;Dicks, Matthew D. J.;Llewellyn, David;Moore, Anne C.;Janse, Chris J.;Franke-Fayard, Blandine M.;Gilbert, Sarah C.;Hill, Adrian V. S.;Pleass, Richard J.;Draper, Simon J.
啮齿动物疟疾种约利疟原虫和夏波迪疟原虫已被广泛用于验证针对血期裂殖子抗原的疫苗方法。然而，越来越多的数据表明，伯氏假体啮齿动物疟疾可能能够绕过疫苗诱导的抗裂殖子反应。在这里，我们证实，尽管使用佐剂蛋白或病毒载体疫苗递送成功地诱导了针对主要merozoite抗原的抗体，但对伯氏假体的保护失败。没有亚单位疫苗方法在小鼠免疫和攻击野生型伯氏疟原虫ANKA或NK65或编码恶性疟原虫merozoite抗原的嵌合寄生虫系后显示有效。在缺乏抑制性Fc受体CD32b的敲除小鼠中，保护作用没有得到改善，也没有对具有非隔离表型的Δsmac伯氏疟原虫系的保护作用。更好地了解对伯氏假体产生保护或未能产生保护的机制，可以指导开发针对恶性疟原虫的有效疫苗。
Rodent malaria species Plasmodium yoelii and P. chabaudi have been widely used to validate vaccine approaches targeting blood-stage merozoite antigens. However, increasing data suggest the P. berghei rodent malaria may be able to circumvent vaccine-induced anti-merozoite responses. Here we confirm a failure to protect against P. berghei, despite successful antibody induction against leading merozoite antigens using protein-in-adjuvant or viral vectored vaccine delivery. No subunit vaccine approach showed efficacy in mice following immunization and challenge with the wild-type P. berghei strains ANKA or NK65, or against a chimeric parasite line encoding a merozoite antigen from P. falciparum. Protection was not improved in knockout mice lacking the inhibitory Fc receptor CD32b, nor against a Δsmac P. berghei parasite line with a non-sequestering phenotype. An improved understanding of the mechanisms responsible for protection, or failure of protection, against P. berghei merozoites could guide the development of an efficacious vaccine against P. falciparum.
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影响因子：
4.4
作者：
de Cassan, Simone C.;Forbes, Emily K.;Draper, Simon J.
通讯作者：
Draper, Simon J.
DOI：
10.1084/jem.20030085
发表时间：
2003-09-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
de Koning-Ward TF;O'Donnell RA;Drew DR;Thomson R;Speed TP;Crabb BS
通讯作者：
Crabb BS
影响因子：
168.9
作者：
Alonso, PL;Sacarlal, J;Cohen, J
通讯作者：
Cohen, J
影响因子：
3.1
作者：
Goodman, Anna L.;Epp, C.;Draper, S. J.
通讯作者：
Draper, S. J.
影响因子：
3.1
作者：
Ahlborg, N;Ling, IT;Riley, EM
通讯作者：
Riley, EM