Allosuppressor and allohelper T cells in acute and chronic graft-vs-host disease. I. Alloreactive suppressor cells rather than killer T cells appear to be the decisive effector cells in lethal graft-vs.-host disease.
Allosuppressor and allohelper T cells in acute and chronic graft-vs-host disease. I. Alloreactive suppressor cells rather than killer T cells appear to be the decisive effector cells in lethal graft-vs.-host disease.
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DOI：
10.1084/jem.155.5.1501
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发表时间：
1982-05-01
期刊：
影响因子：
--
通讯作者：
Gleichmann E
中科院分区：
文献类型：
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作者：
Rolink AG;Radaszkiewicz T;Pals ST;van der Meer WG;Gleichmann E
将来自B10供体的脾T细胞注射到经辐照的（B10 x DBA/2）F1小鼠中。无论是5或6天后，激活的供体T细胞回收这些主要F1（1度F1）受体的脾脏，并转移到组的非辐照同基因F1（2度F1）受体。而第5天激活的亲本T细胞诱导急性移植物抗-在宿主疾病（GVHD）和最终致死的GVHD中，第6天活化的B10 T细胞不能诱导急性GVHD，但诱导慢性GVHD的症状。有趣的是，第6天活化的T细胞不能诱导致死性GVHD不能归因于缺乏抗F1 T杀伤细胞。功能研究的综合结果表明，第6天的细胞富含同种异体反应性辅助T细胞，而第5天的细胞富含同种异体反应性抑制细胞。因此，我们的研究结果表明，急性GVHD和致死性GVHD是由同种异体反应性供体T抑制细胞而不是T杀伤细胞引起的，慢性GVHD的症状是由同种异体反应性供体T辅助细胞引起的。
Splenic T cells from B10 donors were injected into irradiated (B10 x DBA/2)F1 mice. Either 5 or 6 d later, activated donor T cells were recovered from the spleens of these primary F1 (1 degree F1) recipients and transferred to groups of nonirradiated syngeneic F1 (2 degrees F1) recipients. Whereas day-5-activated parental T cells induced the characteristic symptoms of acute graft-vs.-host disease (GVHD) and eventually lethal GVHD, day-6-activated B10 T cells failed to induce acute GVHD but induced symptoms of chronic GVHD. Interestingly, the inability of day-6-activated T cells to induce lethal GVHD could not be ascribed to a lack in anti-F1 T killer cells. The combined results of functional studies indicated that day-6 cells were enriched for alloreactive helper T cells, whereas day-5 cells were enriched for alloreactive suppressor cells. Hence, our findings indicate that acute GVHD and lethal GVHD are caused by alloreactive donor T suppressor but not T killer cells, and that symptoms of chronic GVHD are caused by alloreactive donor T helper cells.