ALTERNATIVE SPLICE FORM OF TYPE-II PROCOLLAGEN MESSENGER-RNA (IIA) IS PREDOMINANT IN SKELETAL PRECURSORS AND NON-CARTILAGINOUS TISSUES DURING EARLY MOUSE DEVELOPMENT
ALTERNATIVE SPLICE FORM OF TYPE-II PROCOLLAGEN MESSENGER-RNA (IIA) IS PREDOMINANT IN SKELETAL PRECURSORS AND NON-CARTILAGINOUS TISSUES DURING EARLY MOUSE DEVELOPMENT
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DOI：
10.1002/aja.1001990206
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发表时间：
1994-02-01
影响因子：
2.5
通讯作者：
REIFE, RA
中科院分区：
文献类型：
--
作者：
SANDELL, LJ;NALIN, AM;REIFE, RA
II型胶原蛋白通常被认为是软骨的特征，在胚胎发生和骨骼发育期间定位于特定的非软骨结构中。II型前胶原以两种不同的形式合成，这两种形式是通过前体mRNA转录物中外显子2的选择性剪接产生的。一种形式（IIA型前胶原）在NH 2-末端前肽中含有一个大的富含半胱氨酸的结构域，而第二种形式（IIB型前胶原）则没有。这两种形式在发育和软骨形成过程中空间表达，其中IIB型前胶原mRNA主要由软骨细胞表达，而IIA型在软骨祖细胞中表达（Sandell等，[1991] J. Cell Biol.114：1307-1319）。本研究表明，早期非软骨的表达，体节，间充质和上皮细胞，主要是交替剪接形式，IIA型前胶原mRNA。在发育后期，IIB型mRNA剪接形式由软骨细胞表达。在膜内骨（如下颌骨）的发育过程中，也表达IIA型前胶原mRNA。在该组织中，剪接形式不转换为IIB型mRNA，并且不形成软骨。这些结果表明，IIA型mRNA的表达，无论是上皮细胞或间充质细胞，形成明显的骨骼结构之前。(C)1994 Wiley-Liss，Inc.
Type II collagen, generally considered to be characteristic of cartilage, has been localized in specific non-cartilaginous structures during embryogenesis and development of the skeleton. Type II procollagen is synthesized in two different forms generated by alternative splicing of exon 2 in the precursor mRNA transcript. One form (type IIA procollagen) contains a large cysteine-rich domain in the NH2-terminal propeptide, while the second form (type IIB procollagen) does not. These two forms are spatially expressed during development and chondrogenesis with the type IIB procollagen mRNA primarily expressed by chondrocytes while the IIA form is expressed in chondroprogenitor cells (Sandell et al. [1991] J. Cell Biol. 114:1307-1319). The present study demonstrates that the early non-cartilage expression, by somites, mesenchymal and epithelial cells, is predominately the alternate splice form, type IIA procollagen mRNA. Later in development, the type IIB mRNA splice form is expressed by chondrocytes. During the development of intramembranous bones, such as the mandible, type IIA procollagen mRNA is also expressed. In this tissue, the splice form does not switch to type IIB mRNA and no cartilage is formed. These results show that expression of type IIA mRNA, whether by epithelial or mesenchymal cells, precedes formation of overt skeletal structures. (C) 1994 Wiley-Liss, Inc.