Bead-based padlock rolling circle amplification for single DNA molecule counting
Bead-based padlock rolling circle amplification for single DNA molecule counting
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DOI：
10.1016/j.ab.2013.02.016
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发表时间：
2013-06-01
影响因子：
2.9
通讯作者：
Nilsson, Mats
中科院分区：
文献类型：
--
作者：
Sato, Kae;Ishii, Reina;Nilsson, Mats
锁式滚环扩增（RCA）是一种非常有效的超灵敏DNA检测方法。尽管基于珠的RCA有一些优点，但尚未报道珠材料与基于珠的RCA的效率之间的关系的详细研究。在这里，我们比较了基于珠的RCA在两种类型的珠材料上进行的反应效率：琼脂糖和聚苯乙烯。琼脂糖是更适合于珠上RCA的材料。校准曲线在0.05和1 nM之间呈线性，沙门氏菌DNA测定的检测限为9 pM（9 μ阿莫尔）。(C)2013 Elsevier Inc. All rights reserved.
Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination. (C) 2013 Elsevier Inc. All rights reserved.