QTL analysis for pseudostem pungency in bunching onion (Allium fistulosum)
QTL analysis for pseudostem pungency in bunching onion (Allium fistulosum)
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DOI：
10.1007/s11032-012-9752-5
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发表时间：
2012-06
影响因子：
3.1
通讯作者：
H. Tsukazaki;S. Yaguchi;K. Yamashita;M. Shigyo;A. Kojima;T. Wako
中科院分区：
文献类型：
--
作者：
H. Tsukazaki;S. Yaguchi;K. Yamashita;M. Shigyo;A. Kojima;T. Wako
低辣度是大葱的重要农艺性状之一。虽然辣度可以通过比色法间接评价，但仍需要与低辣度数量性状基因座（QTL）连锁的DNA标记。在这项研究中，我们通过6个试验进行了3年多的F2：3群体的洋葱假茎的辣味评价。基于遗传连锁图的QTL分析表明，主效辣度QTL位于2a染色体上24.2 cM的区间内。AFAT04B03是一个与辣度QTL连锁的简单重复序列位点，其低辣度等位基因在洋葱栽培品种中很少。此外，在AFAT04B03处的低刺激性亲本衍生等位基因纯合的个体比纯合或杂合的个体显著较不刺激。因此，AFAT 04 B03是一个有效的低辣度选择标记。
Low pungency is one of the most important agronomic traits in bunching onion (Allium fistulosumL.). Although the degree of pungency can be evaluated indirectly using a colorimetric test for pyruvic acid, DNA markers linked to low-pungency quantitative trait loci (QTLs) are still desired. In this study, we evaluated pungency in the bunching onion pseudostem through six trials conducted over 3 years using anF2:3population. QTL analysis based on the genetic linkage map revealed that the major pungency QTL was located within a 24.2-cM interval on Chr. 2a. The low-pungency parent-derived allele at AFAT04B03, a simple sequence repeat locus linked to the pungency QTL, was rare among commercial bunching onion cultivars. In addition, individuals homozygous for the low-pungency parent-derived allele at AFAT04B03 were significantly less pungent than those that were homozygous or heterozygous. Thus, these findings suggest that AFAT04B03 is an effective selection marker for low pungency in bunching onion breeding.