Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine
Flower-like gold nanoparticles-based immunochromatographic test strip for rapid simultaneous detection of fumonisin B1 and deoxynivalenol in Chinese traditional medicine
复制标题
花状金纳米颗粒免疫层析试纸条同时快速检测中药中伏马菌素B-1和脱氧雪腐镰刀菌烯醇
DOI：
10.1016/j.jpba.2019.112895
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发表时间：
2020-01-01
影响因子：
3.4
通讯作者：
Huang, Zibing
中科院分区：
文献类型：
--
作者：
Huang, Xinyu;Huang, Tng;Huang, Zibing
研制了一种基于花状金纳米颗粒（FGN）的免疫层析试纸条（ICS），并首次应用于中药中伏马菌素B-1（FB 1）和脱氧雪腐镰刀菌烯醇（DON）的快速同时检测。对影响ICS灵敏度的几个实验条件进行了研究，包括FGN的类型、FGN-单克隆抗体（MAb）偶联物的制备条件以及ICS的工艺参数。在最佳实验条件下，检测限为5.0ng/mL（对应于中药样品中50 μ g/kg），可在5 min内完成检测。此外，采用高效液相色谱（HPLC）或酶联免疫吸附试验（ELISA）对天然样品进行分析，这些方法的结果与ICS的结果具有良好的相关性。该方法灵敏、快速、方便，可用于大量样品的现场检测。(C)2019 Elsevier B. V.版权所有。
A flower-like gold nanoparticles (FGN)-based immunochromatographic test strip (ICS) was developed and used for the first time for the rapid simultaneous detection of fumonisin B-1 (FB1) and deoxynivalenol (DON) in Chinese traditional medicine. Several experimental conditions affecting the sensitivity of ICS have been investigated, including the type of FGN, the preparation conditions of FGN-monoclonal antibody (MAb) conjugates, and the process parameters of ICS. Under the optimal experimental conditions, the visual limit of detection was 5.0 ng/mL (corresponding to 50 mu g/kg in Chinese traditional medicine samples) for both FB1 and DON, and detection can be completed within 5 min. In addition, the natural samples were analyzed using high-performance liquid chromatography (HPLC) or enzyme-linked immunosorbent assay (ELISA), and the results of these methods showed good correlation with those obtained using ICS. The procedure using FGN-based simultaneous ICS was sensitive, rapid, and convenient for on-site detection of a large number of samples. (C) 2019 Elsevier B.V. All rights reserved.