Identification of Unusual Oxysterols Biosynthesised in Human Pregnancy by Charge-Tagging and Liquid Chromatography - Mass Spectrometry

Identification of Unusual Oxysterols Biosynthesised in Human Pregnancy by Charge-Tagging and Liquid Chromatography - Mass Spectrometry
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通过电荷标记和液相色谱-质谱法鉴定人类妊娠期间生物合成的异常氧甾醇

DOI：
10.1101/2022.02.07.478301
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发表时间：
2022
期刊：
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影响因子：
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通讯作者：
Dickson A
Dickson A
中科院分区：
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文献类型：
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作者：
Dickson A

文献摘要

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本研究的目的是鉴定胎盘、脐带血血浆、母体血浆和羊水中的氧甾醇和任何下游代谢物，以提高我们对这些分子在妊娠中的作用的认识。我们证实了人类胎盘中20s -羟基胆固醇的鉴定，并提出了一个从22r -羟基胆固醇到c27胆汁酸的可能结构为3β，20R， 22r -三羟基胆固醇-5-en-(25R)26-酸的途径。该途径不仅在胎盘中明显，在脐带血浆、母体血浆和羊水中也发现了该途径的中间体，但在非孕妇中不存在。
The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.