Calcium ionophore-induced egg activation and apoptosis are associated with the generation of intracellular hydrogen peroxide
Calcium ionophore-induced egg activation and apoptosis are associated with the generation of intracellular hydrogen peroxide
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DOI：
10.1080/10715760701868352
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Shrivastav, T. G.
中科院分区：
文献类型：
--
作者：
Chaube, S. K.;Khatun, Sabana;Shrivastav, T. G.
本研究旨在探讨钙离子载体诱导的卵细胞激活和凋亡是否与体外培养的大鼠卵细胞产生过氧化氢(H_2O_2)有关。中期Ⅱ(M-II)滞留卵在缺乏钙镁离子的培养液中培养不能诱导卵子激活，而在添加钙镁离子的培养液中培养有20%的卵子出现第二极体。在Ca~(2+)/Mg~(2+)缺乏的条件下，低浓度的钙离子载体(0.2、0.4和0.8微米)不仅以剂量依赖的方式诱导卵细胞激活，而且细胞内H_2O_2的产生(84.40+/-0.50 ng/卵)高于对照组(80.46+/-1.34 ng/卵)。较高浓度的钙离子载体(1.6微米)可诱导卵细胞凋亡，并显著产生细胞内H_2O_2(92.43+/-0.93 ng/卵)。相反，细胞外抗氧化剂如2(3)-叔丁基-4-羟基苯甲醚(BHA)可降低细胞内H_2O_2水平(81.20+/-1.42 ng/个鸡蛋)，并保护细胞免受钙离子载体诱导的卵细胞激活和凋亡的形态变化。这些结果清楚地表明，钙离子载体诱导的激活和凋亡与大鼠卵细胞内过氧化氢的产生有关。
The present study was designed to investigate whether calcium ionophore-induced activation and apoptosis are associated with the generation of hydrogen peroxide (H2O2) in rat eggs cultured in vitro. Culture of metaphase-II (M-II) arrested eggs in Ca2+/Mg2+-deficient medium did not induce egg activation, while a second polar body was observed in 20% of eggs when cultured in Ca2+/Mg2+-supplemented medium. In Ca2+/Mg2+-deficient medium, lower concentrations of calcium ionophore (0.2,0.4 and 0.8 mu m) not only induced egg activation in a dose-dependent manner but also generation of intracellular H2O2 (84.40 +/- 0.50 ng/egg) when compared to control eggs (80.46 +/- 1.34 ng/egg). The higher concentration of calcium ionophore (1.6 mu m) induced apoptosis and pronounced generation of intracellular H2O2 (92.43 +/- 0.93 ng/egg) in treated eggs. Conversely, cell-permeant antioxidant such as 2(3)-tert-butyl-4-hydroxyanisole (BHA) reduced intracellular H2O2 level (81.20 +/- 1.42 ng/egg) and protected against calcium ionophore-induced morphological changes characteristics of egg activation and apoptosis. These results clearly suggest that calcium ionophore-induced activation and apoptosis are associated with the generation of intracellular H2O2 in rat eggs.