PscF is a major component of the Pseudomonas aeruginosa type III secretion needle
PscF is a major component of the Pseudomonas aeruginosa type III secretion needle
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DOI：
10.1016/j.femsle.2005.09.028
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发表时间：
2005-12-01
影响因子：
2.1
通讯作者：
Attree, I
中科院分区：
文献类型：
--
作者：
Pastor, A;Chabert, J;Attree, I
铜绿假单胞菌是一种革兰氏阴性机会致病菌，可将外酶 (Exo) 直接转移到真核细胞的细胞质中。这是通过 III 型分泌/易位机制来完成的。在这里，我们证明铜绿假单胞菌 III 型分泌针结构主要由 PscF 组成，PscF 是分泌和铜绿假单胞菌细胞毒性所需的蛋白质。部分纯化的针状结构与细菌表面分离，长度为 60-80 nm，宽度为 7 nm，类似于耶尔森菌属的针状结构。小肠结肠炎耶尔森氏菌的 YscF 能够在功能上补充 pscF 缺失，但需要在 N 末端有 11 个铜绿假单胞菌特异性氨基酸才能发挥其功能。 (c) 2005 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, translocates exoenzymes (Exo) directly into the eukaryotic cell cytoplasm. This is accomplished by a type III secretion/translocation machinery. Here, we show that the P. aeruginosa type III secretory needle structure is composed essentially of PscF, a protein required for secretion and P. aeruginosa cytotoxicity. Partially purified needles, detached from the bacterial surface, are 60-80 nm in length and 7 nm in width, resembling needles from Yersinia spp.. YscF of Yersinia enterocolitica was able to functionally complement the pscF deletion, but required 11 P. aeruginosa-specific amino acids at the N-terminus for its function. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.