Ultrafast Photoinduced Electron Transfer in a Photosensitizer Protein
Ultrafast Photoinduced Electron Transfer in a Photosensitizer Protein
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DOI：
10.31635/ccschem.021.202100823
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发表时间：
2022
期刊：
影响因子：
11.2
通讯作者：
Wang Jiangyun
中科院分区：
文献类型：
--
作者：
Zheng Dandan;Tao Min;Yu Longjiang;Liu Xiaohong;Xia Andong;Wang Jiangyun
Photosystem II (PSII) is a large membrane protein (~700 kDa) complex, harboring P680+,the strongest oxidant known in biological systems, which is responsible for driving tyrosine oxidation and ultimately 02 generation. While the enhancement and expansion of PSII functions through genetic engineering would be beneficial for driving challenging chemical reactions, this has proven difficult due to its enormous complexity. Here, we report a genetically encoded, 27 kDa photosensitizer protein (PSP3) that recapitulates the initial photoinduced key properties of PSII. Through the genetic incorporation of benzophenone-alanine (BpA) into a fluorescent protein coupled with femtosecond transient absorption measurement, we show that photoinduced electron transfer from residue Tyr203 to the PSP3 chromophore occurs very rapidly (~1 ps),which is comparable with that of the first electron transfer step in PSII. Since PSP3 can be overexpressed in high yield in Escherichia coli and genetically engineered easily, it might facilitate challenging oxidation and reduction reactions in vitro and in vivo.
