Strain dependence of airway hyperresponsiveness reflects differences in eosinophil localization in the lung
Strain dependence of airway hyperresponsiveness reflects differences in eosinophil localization in the lung
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DOI：
10.1152/ajplung.2001.281.2.l394
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发表时间：
2001-08-01
影响因子：
4.9
通讯作者：
Gelfand, EW
中科院分区：
文献类型：
--
作者：
Takeda, K;Haczku, A;Gelfand, EW
不同品系小鼠在卵清蛋白致敏和气道刺激后表现出不同程度的气道高反应性。BALB/c小鼠的抗体反应远远超过C57BL/6小鼠；相比之下，尽管BALB/c小鼠对甲胆碱的反应性要高得多，但C57BL/6小鼠支气管肺泡灌洗液中的嗜酸性粒细胞数量要高得多。致敏和激发BALB/c小鼠吸入甲胆碱或5-羟色胺后肺阻力增加，动态顺应性降低。C57BL/6小鼠仅在监测吸入5-羟色胺的动态依从性时表现出显著的反应性水平。嗜酸性粒细胞在BALB/c小鼠的支气管周围和肺外周组织中积累，而在C57BL/6小鼠的肺外周组织中弥漫性分布。因此，除了抗体反应和胆碱能激动剂反应的差异外，大、小气道反应的差异可能反映了嗜酸性粒细胞在肺组织中的选择性分布。
Different strains of mice exhibit different degrees of airway hyperresponsiveness after sensitization to and airway challenge with ovalbumin. Antibody responses in BALB/c mice far exceeded those in C57BL/6 mice; in contrast, although responsiveness to methacholine was much higher in the BALB/c mice, the number of eosinophils in the bronchoalveolar lavage fluid was higher in C57BL/6 animals. Sensitized and challenged BALB/c mice developed increases in lung resistance and decreases in dynamic compliance after methacholine or 5-hydroxytryptamine inhalation. C57BL/6 mice only exhibited significant levels of responsiveness when dynamic compliance was monitored in response to inhaled 5-hydroxytryptamine. Eosinophils accumulated in the peribronchial and peripheral lung tissue in BALB/c mice but were distributed diffusely in the peripheral lung tissue of C57BL/6 mice. Thus, in addition to differences in antibody responses and cholinergic agonist reactivity, differences in the responses of large and small airways may reflect the selective distribution of eosinophils in lung tissue.